摘要
番杏[Tetragonia tetragonoides(Pall.)Kuntze]是一种抗逆性极强的海水蔬菜。植物类甜蛋白[thaumatin-like protein,(TLP)]基因参与调控多种生物和非生物胁迫应答。为了探究番杏TtTLP基因的抗逆功能,本研究克隆了一个番杏类甜蛋白基因(TtTLP11),并在模式植物拟南芥中进行转基因超表达,获得T3代转基因植株,以转TtTLP11基因拟南芥和野生型拟南芥为试验材料,进行高盐、高渗、高温胁迫和盐碱混合胁迫处理,通过测定种子萌发、幼苗生长状况和检测转基因植株的抗逆性,明确番杏TtTLP11基因的抗逆性功能并探讨可能的作用机制。结果表明:在多种非生物胁迫条件下,TtTLP11基因过表达可减轻胁迫对转基因拟南芥种子萌发的抑制,缓解幼苗受到的伤害,并提高植物的抗逆性。推测该基因可能通过缓解植物体内渗透压剧烈变化,维持细胞内水分平衡,增强植物对非生物胁迫的耐受性。本研究结果为深入解析植物缓解非生物胁迫侵害的分子调控网络提供一定的理论依据。
Tetragonia tetragonoides(Pall.)Kuntze is a seawater vegetable with strong tolerance to multiple abiotic stresses.Plant thaumatin-like proteins(TLPs)are involved in various biological and abiotic stress responses.In order to elucidate the possible roles of a T.tetragonoides thaumatin-like protein gene(TtTLP11)in abiotic stress resistance,we cloned this gene and performed transgenic over-expression assay in Arabidopsis thaliana,then the homozygotic transgenic lines were obtained and performed subsequent analysis for stresses tolerance.In this study,the seeds from transgenic plants over-expressing TtTLP11 and wild type Arabidopsis plants were challenged with high salinity,high osmotic stress,heat and mixed salt-alkali stresses,by detecting the seed germinating rates,seedling growth status,thereby assessing the stress resistance of these transgenic Arabidopsis plants.The purpose of this study was to clarify the abiotic stress resistance functions of the TtTLP11 and the possible molecular mechanisms.The results showed that under various abiotic stress conditions,the over-expression of TtTLP11 could alleviate the inhibition effects of seed germination and relieve the environmental damage to seedlings,thereby improving the abiotic stress tolerance of transgenic plants.It is speculated that TtTLP11 might alleviate dramatic changes in osmotic pressure in plants and maintain water homeostasis,and then affect the stress tolerance of plants.The results could provide a theoretical basis for further analysis of the molecular regulatory network of plant response to alleviate abiotic stress damage.
作者
黄增旺
谢富英
简曙光
黎庭耀
张美
HUANG Zengwang;XIE Fuying;JIAN Shuguang;LI Tingyao;ZHANG Mei(South China Botanical Garden,Chinese Academy of Sciences/Provincial Key Laboratory of Applied Botany,Guangzhou,Guangdong 510650,China;University of Chinese Academy of Sciences,Beijing 100049,China;Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640,China)
出处
《热带作物学报》
北大核心
2025年第10期2335-2345,共11页
Chinese Journal of Tropical Crops
基金
广东省科技计划项目(No.2024B1212050007)。
关键词
番杏
类甜蛋白基因
非生物胁迫
转基因拟南芥
Tetragonia tetragonoides(Pall.)Kuntze
TLP
abiotic stresses
transgenic Arabidopsis
