摘要
旨在探究超数排卵处理后滩羊血浆类固醇激素变化与超排效果之间的关系。本研究对33只经产年龄在4.5±0.1岁的健康滩羊采用CIDR+FSH+PG法超数排卵,采集试验滩羊在人工授精(AI)当天(第0天)和采胚日(第6天)的血液样品并分离血浆,依据采胚日观察到卵巢黄体数量结合样品采集时间将血浆样品为4组,AI当天高反应组(HCA)、AI当天低反应组(LCA)、采卵日高反应组(HC6)、采卵日低反应组(LC6),每组各8个样品。采用液相色谱串联质谱(LC-MS/MS)方法,靶向检测血浆中类固醇激素的变化。结果显示,采卵日滩羊血浆中孕酮(P4)、孕烷二醇(PdG)、去氧皮质酮(DOC)和2-甲氧基雌酮(2-ME)极显著高于AI当天,而AI当天(LCA和HCA组)血浆中4-甲氧基雌酮(4-ME)极显著的高于采卵日;对于AI当天,HCA组17α-羟孕酮浓度(FC=1.50)显著高于LCA组(P<0.05);采卵日两组中,HC6组孕酮(FC=0.52)和去氧皮质酮(FC=0.50)浓度显著高于LC6组(P<0.05),且孕酮和去氧皮质酮浓度与黄体数和总胚胎数之间呈显著强的正相关。结果表明,超数排卵处理后,滩羊血浆类固醇激素发生了变化,采卵日孕酮与去氧皮质酮浓度影响超数排卵效果。
This study aimed to explore the relationship between changes in plasma steroid hormones and superovulation effects in Tan sheep after superovulation treatment.Thirty-three healthy multiparous Tan sheep aged 4.5±0.1 years were subjected to superovulation using the CIDR+FSH+PG method.Blood samples were collected on the day of artificial insemination(AI,Day 0)and embryo collection(Day 6),and plasma was separated.Plasma samples were divided into 4 groups based on the number of ovarian corpora lutea observed on the embryo collection day and the sampling time:high-response group on AI day(HCA),low-response group on AI day(LCA),high-response group on embryo collection day(HC6),and lowresponse group on embryo collection day(LC6),with 8 samples in each group.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)was used to target and detect changes in plasma steroid hormones.The results showed that progesterone(P4),pregnanediol(PdG),deoxycorticosterone(DOC)and(2-methoxyestrone)(2-ME)in plasma of Tan sheep on embryo collection day were significantly higher than those on the day of AI,while 4-methoxyestrone(4-ME)in plasma on the day of AI(LCA and HCA groups)was significantly higher than that on the day of egg collection;The concentration of 17α-hydroxyprogesterone in the HCA group(FC=1.50)was significantly higher than that in the LCA group(P<0.05).The concentrations of progesterone(FC=0.52)and deoxycorticosterone(FC=0.50)in the HC6 group were significantly higher than those in the LC6 group(P<0.05).Additionally,the concentrations of progesterone and deoxycorticosterone on the embryo collection day(6 d)showed a strong correlation with the number of corpora lutea and the total number of embryos.The results indicate that after superovulation treatment,plasma steroid hormones in Tan sheep change,and the concentrations of progesterone and deoxycorticosterone affect the superovulation effect on the embryo collection day.
作者
邢洲
宋承磊
曹凤凤
李卓颖
李庆云
陶金忠
XING Zhou;SONG Chenglei;CAO Fengfeng;LI Zhuoying;LI Qingyun;TAO Jinzhong(College of Animal Science and Technology,Ningxia University,Yinchuan 750021,China)
出处
《畜牧兽医学报》
北大核心
2025年第9期4422-4431,共10页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
宁夏回族自治区重点研发项目(2022BBF03022)。
关键词
滩羊
超数排卵
黄体数
类固醇激素
Tan sheep
superovulation
corpus luteum number
steroid hormone
