摘要
针对胶原基角膜修复材料力学性能不好而导致的不耐缝合以及稳定性不足的问题,尝试将丝素蛋白引入到胶原(Col)基角膜修复材料的内部。首先对天然丝素纤维进行羧基化处理,随后将羧基化丝素蛋白(CSF)引入到胶原凝胶体系,并通过1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐和N-羟基琥珀酰亚胺对二者进行化学交联,成功制备了一种具备“钢筋混凝土”结构的胶原-羟基化丝素分子Col-CSF复合支架。通过红外、热重分析和力学性能测试等对材料进行表征。结果表明,CSF的引入显著提高了薄膜的力学性能,Col-CSF的抗张强度达到(12.66±0.11)MPa。此外,材料还具有良好的热稳定性和透光性能。体外细胞实验结果证明,Col-CSF无细胞毒性,有利于角膜上皮细胞的黏附与生长。
Objective Corneal repair materials refer to artificial or biological substitutes designed to restore damaged corneal tissue.An ideal corneal repair material must exhibit excellent biocompatibility,mechanical stability,and optimal optical characteristics.Current research indicates that while pure collagen-based scaffolds demonstrate promising biological properties,they often suffer from inadequate mechanical strength.Although this limitation can be partially addressed by incorporating polymeric components,such modifications may compromise other critical parameters,including optical clarity and controlled degradation behavior.To address these challenges,the present study proposes the use of natural silk fibroin(SF)fibers as functional reinforcing elements.This approach is expected to provide innovative solutions for the development of advanced collagen-based corneal repair materials with balanced performance characteristics.Method This study first carboxylated natural silk fibroin(CSF)fibers and then introduced it into collagen(Col)system.Col-CSF composite scaffold with"reinforced concrete"structure was successfully prepared by chemical crosslinking of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride(EDC)and n-hydroxysuccinimide(NHS).Through precisely designed chemical modification strategies,we aim to achieve molecular-level integration and synergistic interaction between silk fibroin and collagen molecules.Results The infrared spectra showed that the C—H telescopic vibrational absorption peak appeared at 2930 cm-1 after carboxylation treatment was weakened compared with that of the filipin protein,and the telescopic vibrational absorption peak of C O appeared at 1730 cm-1,and the characteristic absorption peak of C—O—C appeared at 1160 cm-1,indicating that modified silk fibroin had been prepared.In the blended film,the N—H stretching vibration absorption peak gradually approached from 3310 cm-1 to 3280 cm-1.It showed that hydrogen bond was formed between two macromolecules in the blended film,leading to redshift of absorption band.After the addition of CSF,the tensile strength as well as the elongation at break of the films reached(12.66±0.11)MPa and(49.80±0.52)%,respectively,which were significantly higher than that of the pure collagen material.Compared with SF,CSF has more—COOH and provides more cross-linking sites for the material,thus increasing the steric hindrance effect of the fiber molecular conformational change.The plots of water absorption,specific surface area change,and thickness change of the three films demonstrated that the Col-CSF film had higher water absorption(93.6±0.9)%and CSF was with more—COOH,and the interaction between—COOH and collagen—NH 2 led to an increase in the degree of cross-linking,which promotes water absorption.Transmittance was taken as one of the key parameters for evaluating the performance of corneal repair materials and it played an important role in assessing the compatibility between the molecules of the blends.It showed that the light transmittance of all materials increased with the increase of wavelength,which was similar to that of human natural corneal tissue.It is worth noting that the maximum transmittance of Col-SF was more than 80%,while the maximum transmittance of Col-CSF reached 95%.Col-CSF fibers with better ordered arrangement effectively improved the light transmittance,and the samples exhibited good optical properties on a macroscopic scale as a result.In addition,Col-CSF also showed good biocompatibility,which is favorable for cell growth,adhesion and proliferation on it.Conclusion In this study,filipin protein was carboxylated and dispersed in a collagen hydrogel system.The two were chemically crosslinked using EDC/NHS to form a composite scaffold with a reinforced concrete structure.The experimental results showed that the saturated water content of the target material was(93.6±0.9)%and the light transmittance was up to 95%,which were similar to or higher than that of the natural cornea.It was also found that the addition of CSF significantly enhanced the mechanical properties of the material,with the tensile strength and elongation at break reaching(12.66±0.11)MPa and(49.80±0.52)%,respectively,which endowed the material with stronger operability and stability.In addition to this,in vitro cellular experiments showed that the composites had good cytocompatibility,with corneal epithelial cells(HCECs)being able to adhere,proliferate and rapidly cover the entire material surface.Therefore,the artificial corneal repair materials with good biocompatibility and light transmission properties prepared in this study provide a new strategy to improve corneal injury and reduce corneal blindness,and have potential future applications in the field of corneal repair engineering.
作者
高闻语
陈诚
奚晓玮
邓林红
刘杨
GAO Wenyu;CHEN Cheng;XI Xiaowei;DENG Linhong;LIU Yang(School of Medical and Health Engineering,Changzhou University,Changzhou,Jiangsu 213164,China;School of Pharmacy&School of Biological and Food Engineering,Changzhou University,Changzhou,Jiangsu 213164,China;Institute of Biomedical Engineering and Health Sciences,Changzhou University,Changzhou,Jiangsu 213164,China)
出处
《纺织学报》
北大核心
2025年第8期1-9,共9页
Journal of Textile Research
基金
国家自然科学项目基金(81900814)。
关键词
丝素纤维改性
丝素蛋白
胶原蛋白
力学性能
角膜修复材料
医用材料
silk fibroin fiber modification
silk fibroin
collagen
mechanical property
corneal repair material
medical material
