摘要
本试验旨在研究饲料中添加桉枝生物炭对大口黑鲈(Micropterus salmoides)生长性能、抗氧化能力及养殖水质的影响。在基础饲料中分别添加0、2.5、5.0、10.0和20.0 g/kg的桉枝生物炭,制作5种等氮等脂试验饲料,分别记为C0、C2.5、C5、C10、C20组。选取初始体重为(13.34±0.02)g的大口黑鲈600尾,随机分成5组,对应饲喂5种试验饲料,每组4个重复,每个重复30尾鱼,开展为期56 d的养殖试验。结果显示:1)各桉枝生物炭添加组(C2.5、C5、C10、C20组)的终末均重和特定生长率均显著高于对照组(C0组)(P<0.05),以C2.5组最高。C2.5组的增重率显著高于其他组(P<0.05),其饲料系数显著低于其他组(P<0.05)。大口黑鲈成活率、肥满度、脏体比、肝体比和肠体比各组间无显著差异(P>0.05)。2)与C0组相比,C10组血清中总抗氧化能力(T-AOC)显著提高(P<0.05);C2.5和C5组血清中过氧化氢酶(CAT)活性显著提高(P<0.05);C10和C20组血清中丙二醛(MDA)含量显著降低(P<0.05)。C2.5、C10和C20组肝脏中CAT活性较C0组显著升高(P<0.05);各桉枝生物炭添加组肝脏中MDA的含量均较C0组显著降低(P<0.05)。与C0组相比,各桉枝生物炭添加组肠道中T-AOC显著升高(P<0.05),同时MDA含量显著降低(P<0.05)。3)随着养殖时间的延长,各组养殖水体中总磷、总氮、氨氮和亚硝酸盐氮含量呈逐渐上升趋势,其中C5组总磷含量和C2.5组总氮含量较其他组上升速度缓慢。在第35~56天,各桉枝生物炭添加组养殖水体中总磷含量均较C0组显著降低(P<0.05)。在第42~56天,与C0组相比,各桉枝生物炭添加组养殖水体中总氮含量均显著降低(P<0.05)。在第14~56天,与C0组相比,各桉枝生物炭添加组养殖水体中亚硝酸盐氮含量显著低于C0组(P<0.05)。综上可知,本试验条件下,饲料中添加2.5~5.0 g/kg桉枝生物炭可提高大口黑鲈的生长性能,增强抗氧化能力,并通过降低养殖水体中总磷、总氮和亚硝酸盐氮含量改善养殖水质。
This experiment aimed to investigate the effects of diets supplemented with eucalyptus branch biochar on growth performance,antioxidant capacity and aquatic water quality of largemouth bass(Micropterus salmoides).Five isonitrogenous and isolipidic experimental diets were formulated by adding 0,2.5,5.0,10.0 and 20.0 g/kg of eucalyptus branch biochar to the basal diet,designated as groups C0,C2.5,C5,C10 and C20,respectively.Six hundred largemouth bass with an initial body weight of(13.34±0.02)g were randomly allocated into five groups(4 replicates per group with 30 fish per replicate)and fed the corresponding experimental diets for a 56-day feeding trial.The results showed as follows:1)the final average body weight and specific growth rate of largemouth bass in the eucalyptus branch biochar addition groups(groups C2.5,C5,C10 and C20)were significantly higher than those in the control group(group C0)(P<0.05),with the highest in the C2.5 group.The weight gain rate of largemouth bass in the C2.5 group was significantly higher than that in other groups(P<0.05),and its feed conversion ratio(FCR)was significantly lower than that in other groups(P<0.05).There were no significant differences in survival rate,condition factor,viscera somatic index,hepatosomatic index and intestine somatic index of largemouth bass among all groups(P>0.05).2)Compared with the C0 group,the serum total antioxidant capacity(T-AOC)of largemouth bass in the group C10 was significantly increased(P<0.05);the activity of serum catalase(CAT)in the groups C2.5 and C5 was significantly increased(P<0.05);the contents of serum malondialdehyde(MDA)in the groups C10 and C20 was significantly decreased(P<0.05).The activity of liver CAT in the groups C2.5,C10 and C20 was significantly higher than that in the group C0(P<0.05);the content of liver MDA in the eucalyptus branch biochar addition groups was significantly lower than that in the C0 group(P<0.05).Compared with the group C0,the T-AOC in the intestine in the eucalyptus branch biochar addition groups was significantly increased(P<0.05),and the content of MDA was significantly decreased(P<0.05).3)With the extension of the culture time,the contents of total phosphorus(TP),total nitrogen(TN),ammonia nitrogen(NH 3-N)and nitrite nitrogen(NO-2-N)in the aquatic water of each group showed a gradual upward trend.Among them,the increasing rates of TP content in group C5 and TN content in group C2.5 were slower than those in other groups.From the 35th to the 56th day,the aquatic water TP content in the eucalyptus branch biochar addition groups was significantly lower than that in the group C0(P<0.05).From the 42nd to the 56th day,the aquatic water TN content in the eucalyptus biochar addition groups was significantly lower than that in the group C0(P<0.05).From the 14th to the 56th day,the aquatic water NO-2-N content in the eucalyptus branch biochar addition groups was significantly lower than that in the group C0(P<0.05).In summary,under the conditions of this experiment,the addition of 2.5 to 5.0 g/kg eucalyptus branch biochar in the diet can improve the growth performance and enhance the antioxidant capacity of largemouth bass,and improve the aquatic water quality by reducing.the contents of TP,TN and NO-2-N in the aquatic water.
作者
袁海宏
胡俊茹
韦木莲
李金鸿
曾志华
黄文
赵红霞
曹俊明
李翔
陈冰
YUAN Haihong;HU Junru;WEI Mulian;LI Jinhong;ZENG Zhihua;HUANG Wen;ZHAO Hongxia;CAO Junming;LI Xiang;CHEN Bing(Guangdong Key Laboratory of Animal Breeding and Nutrition,Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture and Rural Affairs,Fisheries Research Center of Guangdong Academy of Agricultural Sciences,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Foshan Academy of Agricultural Sciences,Foshan 528000,China;Institute of Agricultural Resources and Environment,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)
出处
《动物营养学报》
北大核心
2025年第9期6163-6175,共13页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
2022年高水平广东省农业科技示范市建设资金市院合作项目(2220060000054)。
关键词
桉枝生物炭
大口黑鲈
生长性能
抗氧化能力
养殖水质
eucalyptus branch biochar
largemouth bass(Micropterus salmoides)
growth performance
antioxidant capacity
aquatic water quality
