摘要
为研究饲料中不同水平绿原酸(CGA)对瓦氏雅罗鱼生长性能、脂代谢、免疫功能及抗氧化能力的影响,本试验采用单因素析因试验设计,将600尾初始体质量为(3.24±0.05)g/尾的瓦氏雅罗鱼随机分为5组,每组3个重复,每个重复放养40尾。5组试验饲料分别添加不同水平[0(CK组)、0.02%(C1组)、0.04%(C2组)、0.06%(C3组)和0.08%(C4组)]的CGA,进行为期8周的饲养试验。结果表明:与CK组相比,添加0.04%~0.06%CGA显著提高生长性能及饲料利用等相关指标(P<0.05);添加0.04%CGA显著提高血清中高密度脂蛋白胆固醇(HDL-C)含量(P<0.05),显著降低血清低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)和总胆固醇(TC)含量(P<0.05),显著升高肝胰脏中脂肪甘油三酯脂酶(ATGL)活性(P<0.05),显著降低肝胰脏中乙酰辅酶A羧化酶(ACC)、ATGL活性和TG含量(P<0.05),显著提高肝胰脏中过氧化物酶体增殖物激活受体-β(PPAR-β)mRNA相对表达量(P<0.05),显著降低肝胰脏中脂肪酸合成酶(FAS)、ACC-1和氧化物酶体增殖物激活受体-γ(PPAR-γ)mRNA相对表达量(P<0.05),促进了血清和肝胰脏中脂肪分解;添加0.04%CGA显著提高血清中免疫球蛋白M(IgM)、补体3(C3)、补体4(C4)含量和溶菌酶(LZM)、碱性磷酸酶(AKP)活性(P<0.05),显著提高肝胰脏中生长转化因子-β(TGF-β)mRNA相对表达量(P<0.05),显著降低肝胰脏中核转录因子-κBp65(NF-κBp65)、髓系分化因子88(MyD88)和肿瘤坏死因子-α(TNF-α)mRNA相对表达量(P<0.05),增强了免疫功能;添加0.04%CGA显著提高血清总抗氧化能力(T-AOC)及过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05),显著降低血清丙二醛(MDA)含量(P<0.05);显著提高肝胰脏中T-AOC及T-SOD、CAT和GSH-Px活性(P<0.05),显著降低肝胰脏中MDA含量(P<0.05);添加0.04%CGA显著提高肝胰脏中核因子红系2相关因子2(Nrf2)、血红素氧合酶-1(HO-1)和CAT mRNA相对表达量(P<0.05),显著降低Kelch样环氧氯丙烷相关蛋白-1(Keap-1)mRNA相对表达量(P<0.05)。综上所述,饲料中添加0.04%CGA可以显著提高瓦氏雅罗鱼生长性能及饲料利用,促进肝胰脏脂肪分解,抑制脂质沉积,通过激活核转录因子信号通路提高免疫功能,激活Keap1-Nrf2信号通路增强抗氧化能力。
To investigate the effects of chlorogenic acid(CGA)levels in diets on growth performance,lipid metabolism,immune function and antioxidant capacity of Leuciscus waleckii,the single-factor factorial design was adopted in this study.A total of 600 fish with an initial body mass of(3.24±0.05)g/fish were randomly divided into 5 groups with 3 replicates per group and 40 fish in each replicate.The 5 groups were fed different levels of CGA[0(CK group),0.02%(C1 group),0.04%(C2 group),0.06%(C3 group)and 0.08%(C4 group)],respectively,and the feeding experiment lasted for 8 weeks.The results showed as follows:compared with CK group,adding 0.04%to 0.06%CGA significantly improved the growth performance and feed utilization of amur ide(P<0.05);adding 0.04%CGA significantly increased serum high-density lipoprotein cholesterol(HDL-C)content(P<0.05),and significantly decreased serum low-density lipoprotein cholesterol(LDL-C),triglyceride(TG)and total cholesterol(TC)contents(P<0.05);it significantly increased the activity of adipose triglyceride lipase(ATGL)in hepatopancreas(P<0.05),and decreased the activities of acetyl coenzyme A carboxylase(ACC)and ATGL and the content of TG in hepatopancreas(P<0.05);it also significantly increased the relative expression of peroxisome proliferator-activated receptor-β(PPAR-β)mRNA in hepatopancreas(P<0.05),and significantly decreased the relative expression levels of fatty acid synthase(FAS),ACC-1 and peroxisome proliferator-activated receptor-γ(PPAR-γ)mRNA in hepatopancreas(P<0.05).It promoted lipolysis in serum and hepatopancreas;the contents of immunoglobulin M(IgM),complement 3(C3)and complement 4(C4)in serum and the activities of lysozyme(LZM)and alkaline phosphatase(AKP)in serum were significantly increased by 0.04%CGA supplementation(P<0.05),and the relative expression level of growth transforming factor-β(TGF-β)mRNA in hepatopancreas was significantly increased(P<0.05);the relative expression level of nuclear factor-κBp65(NF-κBp65),myeloid differentiation factor 88(MyD88)and tumor necrosis factor-α(TNF-α)mRNA in hepatopancreas was significantly reduced(P<0.05),and the immune function was enhanced;adding 0.04%CGA significantly increased serum total antioxidant capacity(T-AOC)and activities of catalase(CAT),total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)(P<0.05),and significantly decreased serum malondialdehyde(MDA)content(P<0.05);It significantly increased the activities of T-AOC,T-SOD,CAT and GSH-Px in hepatopancreas(P<0.05),and decreased the content of MDA in hepatopancreas(P<0.05);addition of 0.04%CGA significantly increased the relative expression levels of nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1)and CAT mRNA in hepatopancreas(P<0.05);it also significantly reduced the relative expression levels of Keap-1 mRNA(P<0.05).In conclusion,dietary supplementation of 0.04%CGA can significantly improve growth performance and feed utilization of amur ide,promote hepatopancreas lipdecomposition,inhibit lipid deposition,enhance immune function by activating nuclear transcription factor signaling pathway and enhance antioxidant capacity by activating Keap1-Nrf2 signaling pathway.
作者
王吟涛
孟思彤
邹瑞兴
张立全
佟娜
吴莉芳
WANG Yintao;MENG Sitong;ZOU Ruixing;ZHANG Liquan;TONG Na;WU Lifang(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;Key Laboratory of Animal Production and Product Quality and Safety,Ministry of Education,Changchun 130118,China;Key Experiments in Animal Nutrition and Feed Science in Jilin Province,Changchun 130118,China;Taipingchi Reservoir Management Center of Nongan County,Changchun 130228,China;Jian City Fisheries Technology Promotion Station,Ji’an 134299,China;Namu Township Comprehensive Service Center,Shuangliao 136400,China)
出处
《动物营养学报》
北大核心
2025年第9期6136-6152,共17页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
吉林省科技厅重点研发项目(20240303084NC)。
关键词
绿原酸
瓦氏雅罗鱼
生长性能
脂代谢
免疫功能
抗氧化能力
chlorogenic acid
Leuciscus waleckii
growth performance
lipid metabolism
immune function
antioxidant capacity
