摘要
本试验旨在研究饲料中添加精氨酸(Arg)对松浦镜鲤幼鱼生长性能、血液指标和肝脏糖代谢的影响。选取360尾健康、初始体重为(6.84±0.02)g的松浦镜鲤幼鱼,随机分为5组,每组4个重复,每个重复18尾。5组试验鱼分别饲喂Arg添加水平为0(C0组)、0.6%(C0.6组)、1.2%(C1.2组)、1.8%(C1.8组)和2.4%(C2.4组)的等氮等能饲料。试验期8周。结果表明:1)与C0组相比,各Arg添加组增重率(WGR)均显著提高(P<0.05),饲料系数(FCR)均显著降低(P<0.05);C1.2、C1.8和C2.4组肥满度(CF)显著提高(P<0.05);C1.8和C2.4组蛋白质效率(PER)显著提高(P<0.05)。基于WGR的折线回归分析表明,松浦镜鲤幼鱼的最适Arg需要量为饲料干物质的1.85%,饲料蛋白质的5.53%。2)与C0组相比,C1.2、C1.8和C2.4组血清甘油三酯(TG)含量显著降低(P<0.05);C2.4组血清总胆固醇(T-CHO)和高密度脂蛋白胆固醇(HDL-C)含量显著降低(P<0.05)。3)C2.4组肝脏葡萄糖激酶(GK)和糖原合成酶激酶3β(GSK3β)活性显著高于其他各组(P<0.05),肝脏磷酸果糖激酶(PFK)活性显著低于其他各组(P<0.05)。与C0组相比,C1.2和C1.8组肝脏己糖激酶(HK)活性显著提高(P<0.05);C1.8和C2.4组肝脏6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶2(PFKFB2)活性显著提高(P<0.05);C1.2、C1.8和C2.4组肝脏胰岛素受体(IR)、磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(AKT)、葡萄糖转运蛋白2(GLUT2)和葡萄糖转运蛋白4(GLUT4)含量显著提高(P<0.05);C1.2组肝脏磷酸化蛋白激酶B(p-AKT)含量显著提高(P<0.05)。4)在肝脏葡萄糖转运相关基因中,与C0组相比,C0.6和C1.2组AKT、各Arg添加组蛋白激酶B底物160(AS160)及C0.6、C1.2和C1.8组GLUT2的mRNA相对表达量均显著提高(P<0.05)。在肝脏糖酵解相关基因中,与C0组相比,C1.2组PFKFB2、C1.2和C1.8组GK及C0.6、C1.2和C1.8组HK的mRNA相对表达量均显著提高(P<0.05)。在肝脏糖异生相关基因中,C1.2、C1.8和C2.4组叉头盒蛋白O1(FOXO1)的mRNA相对表达量显著高于C0和C0.6组(P<0.05);与C0组相比,C2.4组葡萄糖6磷酸酶(G6Pase)及各Arg添加组磷酸烯醇式丙酮酸羧激酶(PEPCK)的mRNA相对表达量均显著降低(P<0.05)。在肝脏糖原合成相关基因中,C0.6和C1.2组糖原合成酶(GSase)的mRNA相对表达量显著高于其他各组(P<0.05)。综上所述,饲料中添加0.6%~2.4%Arg可提高松浦镜鲤幼鱼的生长性能及促进肝脏糖代谢。
This experiment was conducted to investigate the effects of dietary arginine(Arg)supplementation on growth performance,blood indexes and hepatic glucose metabolism of juvenile Songpu mirror carp.A total of 360 healthy juvenile Songpu mirror carp with an initial body weight of(6.84±0.02)g were randomly divided into 5 groups with 4 replicates per group and 18 fish per replicate.The fish in 5 groups were fed isonitrogenous and isoenergetic diets supplemented with 0(C0 group),0.6%(C0.6 group),1.2%(C1.2 group),1.8%(C1.8 group)and 2.4%(C2.4 group)Arg,respectively.The trial lasted for 8 weeks.The results showed as follows:1)compared with the C0 group,the weight gain rate(WGR)in all Arg-supplemented groups was significantly increased(P<0.05),and the feed conversion ratio(FCR)was significantly decreased(P<0.05);the condition factor(CF)in the C1.2,C1.8 and C2.4 groups was significantly increased(P<0.05);the protein efficiency ratio(PER)in the C1.8 and C2.4 groups was significantly increased(P<0.05).Broken line regression analysis based on WGR indicated that the optimal dietary Arg requirement for juvenile Songpu mirror carp was 1.85%of dry matter or 5.53%of dietary protein.2)Compared with the C0 group,the content of triglyceride(TG)in serum in the C1.2,C1.8 and C2.4 groups was significantly decreased(P<0.05);the contents of total cholesterol(T-CHO)and high-density lipoprotein cholesterol(HDL-C)in serum in the C2.4 group were significantly decreased(P<0.05).3)The activities of hepatic glucokinase(GK)and glycogen synthase kinase 3β(GSK3β)in the C2.4 group were significantly higher than those in other groups(P<0.05),while the activity of hepatic phosphofructokinase(PFK)was significantly lower than that in other groups(P<0.05).Compared with the C0 group,the activity of hepatic hexokinase(HK)in the C1.2 and C1.8 groups was significantly increased(P<0.05);the activity of hepatic 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2(PFKFB2)in the C1.8 and C2.4 groups was significantly increased(P<0.05);the contents of hepatic insulin receptor(IR),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),glucose transporter 2(GLUT2)and glucose transporter 4(GLUT4)in the C1.2,C1.8 and C2.4 groups were significantly increased(P<0.05);and the content of hepatic phosphorylated protein kinase B(p-AKT)in the C1.2 group was significantly increased(P<0.05).4)Among hepatic genes related to glucose transport,compared with the C0 group,the mRNA relative expression levels of AKT in the C0.6 and C1.2 groups,protein kinase B substrate 160(AS160)in all Arg-supplemented groups,and GLUT2 in the C0.6,C1.2 and C1.8 groups were significantly increased(P<0.05).Among hepatic genes related to glycolysis,compared with the C0 group,the mRNA relative expression levels of PFKFB2 in the C1.2 group,GK in the C1.2 and C1.8 groups,and HK in the C0.6,C1.2 and C1.8 groups were significantly increased(P<0.05).Among hepatic genes related to gluconeogenesis,the mRNA relative expression level of forkhead box protein O1(FOXO1)in the C1.2,C1.8 and C2.4 groups was significantly higher than that in the C0 and C0.6 groups(P<0.05);compared with the C0 group,the mRNA relative expression levels of glucose-6-phosphatase(G6Pase)in the C2.4 group and phosphoenolpyruvate carboxykinase(PEPCK)in all Arg-supplemented groups were significantly decreased(P<0.05).Among hepatic genes related to glycogen synthesis,the mRNA relative expression level of glycogen synthase(GSase)in the C0.6 and C1.2 groups was significantly higher than that in other groups(P<0.05).In conclusion,dietary supplementation with 0.6%to 2.4%Arg can improve the growth performance and promote hepatic glucose metabolism of juvenile Songpu mirror carp.
作者
李晋南
吴迪
范泽
王连生
王常安
刘红柏
韩世成
LI Jinnan;WU Di;FAN Ze;WANG Liansheng;WANG Chang’an;LIU Hongbai;HAN Shicheng(Key Laboratory of Aquatic Animal Diseases and Immune Technology of Heilongjiang Province,Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China)
出处
《动物营养学报》
北大核心
2025年第9期6110-6121,共12页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
中央级公益性科研院所基本科研业务费专项(HSY202405M)
国家重点研发计划项目(2023YFD2400405)
黑龙江省博士后科学基金(LBH-Z22040)
中国水产科学研究院中央级公益性科研院所基本科研业务费专项基金资助(2023TD60)
国家自然科学基金项目(31702353)
黑龙江省优秀青年基金项目(YQ2019C035)。
关键词
松浦镜鲤
精氨酸
生长性能
血液指标
糖代谢
Songpu mirror carp
arginine
growth performance
blood indexes
glucose metabolism
