摘要
目的探讨微小RNA-145-5p(miR-145-5p)、肌成束蛋白-1(fascin actin-bundling protein-1,FSCN1)在胃癌组织中的表达及生存预测价值。方法选取邯郸市第一医院2019年8月—2021年8月收治的胃癌患者103例。对患者癌组织及癌旁组织FSCN1mRNA、miR-145-5p表达进行检测并分析,使用Pearson分析评估miR-145-5p、FSCN1 mRNA表达的相关性,受试者工作特征曲线下面积(area under the curve,AUC)评估miR-145-5p、FSCN1 mRNA对胃癌患者预后的预测价值;Cox比例风险回归模型对不良预后影响因素进行分析,并采用Kaplan-Meier法进行生存分析。结果与癌旁组织比较,癌组织的miR-145-5p表达水平降低(P<0.05),FSCN1 mRNA表达升高(P<0.05)。与高分化、肿瘤分期I+II期、浸润深度T1~T2、无淋巴结转移、肿瘤最大直径<5 cm癌组织比较,低中分化、肿瘤分期III+IV期、浸润深度T3~T4、有淋巴结转移、肿瘤最大直径≥5 cm的癌组织miR-145-5p表达水平降低,而FSCN1 mRNA表达升高(P<0.05)。Pearson分析显示,miR-145-5p与FSCN1 mRNA表达在患者癌组织中为负相关关系(r=-0.617,P=0.000)。COX多因素回归分析表明,低中分化、肿瘤分期III+IV期、肿瘤最大直径≥5 cm、浸润深度T3~T4、miR-145-5p低表达、FSCN1 mRNA高表达、有淋巴结转移是胃癌不良预后的危险因素(P<0.05)。Kaplan-Meier生存曲线表明,miR-145-5p低表达组与miR-145-5p高表达组3年总生存率差异有统计学意义(P<0.05)。FSCN1 mRNA低表达组与FSCN1 mRNA高表达组3年总生存率差异有统计学意义(P<0.05)。ROC曲线分析结果显示,miR-145-5p、FSCN1 mRNA联合检测的AUC、灵敏度分别为0.947、92.3%,均显著高于单项指标的AUC、灵敏度(P<0.05)。结论miR-145-5p、FSCN1与胃癌患者的临床病理特征有密切关系,是其生存预后有效预测指标。
Objective To explore the expression and prognostic value of microRNA-145-5p(miR-145-5p)and fascin actin-bundling protein-1(FSCN1)in gastric cancer tissues.Methods The study participants were selected from 103 gastric cancer patients treated at The First Hospital of Handan from August 2019 to August 2021.The expression levels of miR-145-5p and FSCN1 mRNA in the cancer tissues and adjacent non-cancerous tissues of gastric cancer patients were measured.Pearson correlation analysis was used to assess the relationship between the expression of miR-145-5p and FSCN1 mRNA.The area under curve(AUC)was used to evaluate the prognostic value of miR-145-5p and FSCN1 mRNA for gastric cancer patients.The Cox proportional hazards regression model was used to analyze factors influencing adverse prognosis,and Kaplan-Meier method was employed for survival analysis.Results The expression level of miR-145-5p was lower in cancer tissues compared to adjacent tissues(P<0.05),while the expression level of FSCN1 mRNA was higher in cancer tissues compared to adjacent tissues(P<0.05).The expression level of miR-145-5p was lower in cancer tissues of the patients with moderate-to-poor differentiation,tumor stage III+IV,invasion depth T3-T4,lymph node metastasis,or maximum tumor diameter≥5 cm,compared to those with high differentiation,tumor stage I+II,invasion depth T1-T2,no lymph node metastasis,or maximum tumor diameter<5 cm,whereas the expression level of FSCN1 mRNA was higher(P<0.05).Pearson analysis revealed a negative correlation between miR-145-5p and FSCN1 mRNA expression in cancer tissues(r=-0.617,P=0.000).COX multivariate regression analysis indicated that moderate-to-poor differentiation,tumor stage III+IV,maximum tumor diameter≥5 cm,invasion depth T3-T4,low miR-145-5p expression,high FSCN1 mRNA expression,and lymph node metastasis were risk factors for poor prognosis in gastric cancer(P<0.05).Kaplan-Meier survival curves demonstrated a statistically significant difference in the 3-year overall survival rate between the low and high miR-145-5p expression groups(P<0.05).Similarly,a statistically significant difference was observed between the low and high FSCN1 mRNA expression groups(P<0.05).ROC curve analysis showed that the combined detection of miR-145-5p and FSCN1 mRNA yielded an AUC of 0.947 and a sensitivity of 92.3%,both of which were significantly higher than those of individual indicators(P<0.05).Conclusion MiR-145-5p and FSCN1 are closely related to the clinical pathological characteristics of gastric cancer patients and are effective prognostic indicators for survival.
作者
薛富森
陈大为
孙江华
XUE Fusen;CHEN Dawei;SUN Jianghua(Comprehensive Surgery Department of Textile Hospital District,The First Hospital of Handan,Handan Hebei 056000,China)
出处
《昆明医科大学学报》
2025年第9期114-120,共7页
Journal of Kunming Medical University
基金
邯郸市科学技术与发展计划项目(23422083289)。
