摘要
目的:采用双样本孟德尔随机化分析方法探讨哮喘与气虚表型之间的关联。方法:哮喘数据集主要来源于Valette K等研究,气虚质主要通过全身去脂重量(Whole Body Fat–free Mass,WBFFM)、虚弱指数(Frailty Index,FI)、近两周内疲劳嗜睡频率(Frequency of Tiredness and Lethargy in Last 2 Weeks,FT)、上呼吸道感染(Upper Respiratory Tract Infection,URTI)、头晕目眩、记忆力减退等主要体质特征表现,FI数据来自Janice L Atkins的研究,WBFFM数据、FT通过IEU Open GWAS数据库下载,URTI、头晕目眩、记忆力减退通过芬兰基因数据库下载。通过R语言Two–Sample MR软件包进行孟德尔随机化分析,将逆方差加权(IVW)方法作为主要分析方法,通过MR–Egger、Weighted median、Simple mode、Weighted mode验证其可靠性,并进行异质性检验、敏感性分析、多效性分析。结果:IVW分析结果表明,哮喘与WBFFM呈负相关(OR=0.988 5,95%CI=0.979 2~0.997 9),与FI(OR=1.117 3,95%CI=1.095 0~1.139 1)、头晕目眩(OR=1.076 0,95%CI=1.017 0~1.138 2)、URTI(OR=1.121 4,95%CI=1.080 9~1.163 3)呈正相关,结果具有显著性。哮喘与记忆力减退(OR=1.015 8,95%CI=0.910 0~1.133 9)、FT(OR=1.010 4,95%CI=0.999 0~1.021 2,P=0.056 1)呈正相关,但结果缺乏显著性。异质性检验提示不存在异质性,敏感性分析均显示稳健,且未显示多效性。结论:哮喘可增加多项气虚表型的风险,在哮喘的诊疗中应及时识别患者是否存在继发性气虚,及时干预,“既病防变”。
Objective:To explore the correlation between asthma and Qi(气)deficiency phenotype by two-sample Mendelian randomization analysis.Methods:The asthma data set was mainly derived from studies conducted by Valette K et al.Qi deficiency constitution type was mainly manifested by main physical characteristics of whole body fat-free mass(WBFFM),frailty index(FI),frequency of tiredness and lethargy in last 2 weeks(FT),upper respiratory tract infection(URTI),dizziness,and memory loss.FI data was obtained from the study of Janice L Atkins,WBFFM and FT data was downloaded from the IEU Open GWAS database,and RUTI,dizziness,and memory loss data was downloaded from the Finnish genetic database.The R language Two-Sample MR software package was used for Mendelian randomization analysis.IVW method was used as the main analysis method,and the reliability was verified by MR-Egger,Weighted median,Simple mode,and Weighted mode.Heterogeneity test,sensitivity analysis,and pleiotropy analysis were performed.Results:The IVW analysis results showed that asthma was negatively correlated with WBFFM(OR=0.9885,95%CI=0.9792~0.9979),and positively correlated with FI(OR=1.1173,95%CI=1.0950~1.1391),dizziness(OR=1.0760,95%CI=1.0170~1.1382),URTI(OR=1.1214,95%CI=1.0809~1.1633),and the results were significant.Asthma was positively correlated with memory loss(OR=1.0158,95%CI=0.9100~1.1339)and FT(OR=1.0104,95%CI=0.9990~1.0212,P=0.0561),but the outcome was not significant.Heterogeneity tests indicated the absence of heterogeneity,sensitivity analyses all showed robustness,and there were no pleiotropy.Conclusion:Asthma can increase the risk of multiple Qi deficiency phenotypes.In the diagnosis and treatment of asthma,it is necessary to promptly identify whether patients have secondary Qi deficiency bias,and timely intervene it when there is a need,so as to prevent disease from changing.
出处
《中医临床研究》
2025年第20期1-10,共10页
Clinical Journal Of Chinese Medicine
基金
安徽省中医药传承创新科研项目(2022CCYB09)
新安医学与中医药现代化研究所“揭榜挂帅”项目(2023CXMMTCM005)
滁州市科技计划项目(2023ZD026)。
关键词
哮喘
气虚
孟德尔随机化
Asthma
Qi deficiency
Mendel randomization
