摘要
目的 分析肝癌组织中长链非编码RNA SBF2-AS1(LncRNA SBF2-AS1)的表达及临床意义,探讨其在肝癌诊疗一体化中的应用价值。方法 选取2021~2022年被广西医科大学第一附属医院收治的原发性肝细胞癌54例患者作为研究对象,收集术后切除的肝癌组织和癌旁组织标本,采集患者的病理组织、临床检验诊断和治疗方式等多项临床治疗资料,采用实时荧光定量PCR(RT-qPCR)技术检测组织标本中的LncRNA SBF2-AS1表达水平,并利用生物统计方法分析其与临床诊断和治疗指标的相关性,运用生物数据库信息分析LncRNA SBF2-AS1对肝癌的诊断价值。结果 肝癌组织中的LncRNA SBF2-AS1的相对表达量(10.656)明显高于癌旁(1.000),差异有统计学意义(Z=-5.675,P<0.001);LncRNA SBF2-AS1的表达与谷丙转氨酶(r=0.297,P<0.05)、中性粒细胞(r=0.275,P<0.05)水平、不同治疗方式(r=0.332,P<0.05)呈正相关性;根据LncRNA SBF2-AS1表达中位值(M=3.649)将患者分为高表达组(27例)和低表达组(27例),LncRNA SBF2-AS1的表达水平与谷丙转氨酶有关(P<0.05)。通过TCGA数据库获取371例肝癌组织及50例癌旁组织的LncRNA SBF2-AS1相对表达量,ROC曲线下面积(AUC)为0.9122,特异度和灵敏度各为94.00%、79.51%。结论 LncRNA SBF2-AS1在肝癌组织中高表达,与谷丙转氨酶、中性粒细胞和治疗方式有关,可成为肝癌诊断及炎性损伤监测的标志物,具有临床诊疗一体化的应用价值。
Objective To analyze the expression and clinical significance of long non⁃coding RNA SBF2⁃AS1(LncRNA SBF2⁃AS1)in hepatocellular carcinoma(HCC)tissues,and to explore its application value in theranostics of HCC.Methods A total of 54 patients with primary HCC admitted to the First Affiliated Hospital of Guangxi Medical University from 2021 to 2022 were selected as the study subjects.Postoperatively resected specimens from HCC and adjacent tissues were collected,along with multiple clinical treatment data,including pathological tissues,clinical laboratory diagnostics and treatment methods.The expression level of LncRNA SBF2⁃AS1 in the tissue specimens were detected by real⁃time quantitative polymerase chain reaction(RTqPCR)and analyzed for correlation with clinical diagnostic and therapeutic indicators using biostatistical methods.Bioinformatics databases were utilized to analyze the diagnostic value of LncRNA SBF2⁃AS1 for HCC.Results The relative expression level of LncRNA SBF2⁃AS1 in HCC tissues(10.656)was significantly higher than that in adjacent tissues(1.000),with a statistically significant difference(Z=-5.675,P<0.001).The expression of LncRNA SBF2⁃AS1 was positively correlated with glutathione aminotransferase(r=0.297,P<0.05),neutrophil(r=0.275,P<0.05),and different treatment methods(r=0.332,P<0.05).Patients were divided into a high expression group(27 patients)and a low expression group(27 patients)based on the median value of LncRNA SBF2⁃AS1 expression(M=3.649),and the expression level of LncRNA SBF2⁃AS1 was correlated with ghrelin transaminase(P<0.05).The relative expression levels of LncRNA SBF2⁃AS1 in 371 cases of l HCC tissue and 50 cases of adjacent tissues were obtained from the cancer genome atlas(TCGA)database.The area under the ROC curve(AUC)was 0.9122,with specificity of 94.00%and sensitivity of 79.51%.Conclusions LncRNA SBF2⁃AS1 is highly expressed in HCC tissues,which is related to alanine aminotransferase,neutrophils and treatment methods,and can serve as a biomarker for HCC diagnosis and inflammatory injury monitoring,demonstrating significant application value in clinical theranostics.
作者
向冬云
赵莲
吴惠仪
孙昊
邓敬桓
XIANG Dongyun;ZHAO Lian;WU Huiyi;SUN Hao;DENG Jinghuan(School of Public Health,Guangxi Medical University,Nanning 530021,China;The First Clinical College of Medicine,Guangxi Medical University,Nanning 530021,China)
出处
《武警医学》
2025年第8期668-673,共6页
Medical Journal of the Chinese People's Armed Police Force
基金
国家自然科学基金(82060434)
2024年广西研究生教育创新计划项目(YCSW2024260)
