摘要
类器官(organoids)作为干细胞自组装形成的三维(3D)微组织模型,被认为是未来生物医药领域的前沿工具。现有类器官仍面临结构简单、功能成熟度不足、异质性高等问题。生物3D打印技术能够实现细胞及生物材料的特定空间位置排布,为复杂类器官构建提供了可能。该文综述了生物3D打印类器官的技术发展,聚焦了打印策略创新、智能生物墨水设计,并结合发育生物学提出了微环境的精确构建策略。最后,展望了类器官生物3D打印技术在组织工程领域的广阔应用前景。
Organoids,3D(three-dimensional)microtissue models formed through the self-organizationof stem cells,are regarded as a frontier tool in biomedical research.However,current organoids still face limitations such as restricted structural complexity,insufficient functional maturity,and high heterogeneity.Bioprintingtechnologies enable precise spatial arrangement of cells and biomaterials,offering new strategies for constructingcomplex organoids.This review summarizes recent advances in organoid bioprinting,with a focus on innovationsin printing strategies and the design of intelligent bioinks,and further proposes approaches for precise microenvironment construction based on developmental biology.Finally,the potential applications and future directions ofbioprinted organoids in tissue engineering and related fields are discussed.
作者
张镌
顾奇
ZHANG Juan;GU Qi(Human Organ Physiopathology Emulation System,State Key Laboratory of Organ Regeneration and Reconstruction,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China;Beijing Institute for Stem Cell and Regenerative Medicine,Beijing 100101,China;Key Laboratory of Animal Cellular and GeneticEngineering of Heilongjiang Province,College of Life Science,Northeast Agricultural University,Harbin 150038,China;University of Chinese Academy of Sciences,Beijing 101408,China)
出处
《中国细胞生物学学报》
2025年第9期2205-2220,共16页
Chinese Journal of Cell Biology
基金
国家重点研发计划(批准号:2025YFC3408800)
中国科学院动物研究所建制化攻关项目(批准号:2023IOZ0101)
中国科学院青年基础研究项目(批准号:YSBR-012)资助的课题。
