摘要
【目的】解析蓝莓R2R3-MYB转录因子家族在类黄酮调控中的表达模式,为探究蓝莓参与类黄酮合成调控机制提供理论基础。【方法】运用生物信息学手段,对蓝莓R2R3-MYB基因的理化性质、亚细胞定位等进行分析;通过RT-qPCR检测VcMYBs基因在不同组织和果实发育期的表达。【结果】在蓝莓中鉴定出278个R2R3-MYB基因,其氨基酸序列长度为160-970 aa;多数成员亚细胞定位在细胞核,进化树分析将VcMYBs分为23个亚家族,同亚家族基因结构特性相似;顺式作用元件分析表明,VcMYBs可能参与生物和非生物胁迫、激素响应、植物生长发育以及光响应;从可能参与类黄酮合成调控的基因(SG4、SG5、SG6和SG7)中随机选择14个,RT-q PCR分析显示,多数VcMYBs基因在果实中表达水平较高,其中,VcMYB91(SG6)和VcMYB56(SG5)等基因在绿果期低表达,成熟期高表达,可能参与果实着色;然而VcMYB88(SG4)和VcMYB96(SG4)等基因在绿果期高表达,成熟期低表达,可能抑制果实着色;VcMYB236(SG7)和VcMYB44(SG7)基因可能参与黄酮醇合成。【结论】全基因组分析鉴定出278个蓝莓R2R3-MYB基因,解析发现SG5/6亚组4个基因(VcMYB56、VcMYB91等)调控花青素合成,SG4/5亚组6个基因(VcMYB88、VcMYB96等)参与原花青素合成,SG7亚组2个基因(VcMYB236、VcMYB44)可能调控黄酮醇代谢。
【Objective】Elucidating the expression patterns of the R2R3-MYB transcription factor family in blueberry during flavonoid regulation,thereby providing a theoretical foundation for exploring the regulatory mechanisms underlying flavonoid biosynthesis in blueberry.【Method】Bioinformatics was used to analyze the characteristics of R2R3-MYB genes in blueberry,such as physicochemical properties and subcellular localization,and RT-qPCR was to detect the expression patterns of VcMYBs in different tissues and different fruit development stages.【Result】A total of 278 R2R3-MYB genes were identified in blueberry,with amino acid sequence lengths ranging from 160 to 970 aa,and most of them were located in the nucleus.The blueberry VcMYBs was divided into 23 subfamilies by phylogenetic tree analysis,and the genes belonging to the same subfamily had similar structural characteristics.Cis-acting element analysis showed that VcMYBs may be involved in biological stress,abiotic stress,hormone induction,plant growth and development,and light response.Fourteen genes(SG4,SG5,SG6 and SG7)that may be involved in the regulation of flavonoid synthesis were randomly selected for further analysis.RT-qPCR analysis showed that the most of the VcMYBs had high expressions in fruits.Among them,VcMYB91(SG6)and VcMYB56(SG5)genes were lowly expressed in the green fruit stage and highly expressed in the mature stage,suggesting that they may be involved in fruit coloring.However,VcMYB88(SG4)and VcMYB96(SG4)showed the opposite expression pattern,suggesting that they may inhibit fruit coloring.In addition,VcMYB236(SG7)and VcMYB44(SG7)genes may be involved in flavonoid synthesis.【Conclusion】The 278 blueberry R2R3-MYB genes were identified via genomewide analysis.Functional dissection revealed that four genes(VcMYB56,VcMYB91,etc.)from the SG5/6 subgroups regulate anthocyanin biosynthesis,six genes(VcMYB88,VcMYB96,etc.)from the SG4/5 subgroups participate in proanthocyanidin synthesis,and two genes(VcMYB236,VcMYB44)from the SG7 subgroup may govern flavonol metabolism.
作者
刘佳丽
宋经荣
赵文宇
张馨元
赵子洋
曹一博
张凌云
LIU Jia-li;SONG Jing-rong;ZHAO Wen-yu;ZHANG Xin-yuan;ZHAO Zi-yang;CAO Yi-bo;ZHANG Ling-yun(State Key Laboratory of Efficient Production of Forest Resources,Key Laboratory of Forest Silviculture and Conservation of the Ministry of Edu-cation,The College of Forestry,Beijing Forestry University,Beijing 100083)
出处
《生物技术通报》
北大核心
2025年第9期124-138,共15页
Biotechnology Bulletin
基金
山东省重点研发计划(2024LZGCQY025)
国家自然科学基金项目(31872942)
北京林业大学“5·5工程”科研创新团队项目(BLRC2023B08)。
