摘要
该研究基于核因子E2相关因子2(Nuclear Factor-erythroid 2-related Factor 2,Nrf2)/血红素氧合酶-1(Heme Oxygenase-1,HO-1)信号通路探讨西洋参多糖(American Ginseng Polysaccharide,AGP)对H_(2)O_(2)诱导HepG2细胞氧化损伤的保护作用及机制。选择H_(2)O_(2)浓度300μmol/L建立HepG2细胞氧化损伤模型(细胞存活率为62.23%),并给予320、640和1280μg/mL的AGP进行干预,分别对各组细胞中活性氧(ROS)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH)、丙二醛(MDA)的水平进行测定;通过Western blot法分析细胞中Nrf2、SOD1、SOD2、HO-1、CAT蛋白的表达。结果表明:AGP对HepG2细胞的增殖无明显作用,AGP三个剂量组均能显著提高氧化损伤HepG2细胞存活率,分别为89.36%、91.42%和91.06%,且明显降低氧化损伤细胞中ROS水平(15.74%、20.71%、25.39%)及MDA含量,对细胞内SOD、GSH、CAT的水平起到有效的提升作用;Western blot结果显示AGP能显著上调Nrf2、SOD1、SOD2、HO-1、CAT等蛋白表达水平(P<0.05,P<0.01)。综上所述,AGP能够通过调控Nrf2/HO-1信号通路改善HepG2细胞氧化损伤,研究结果可为AGP的进一步开发利用提供更多的数据支持。
In this study,the protective effect and underlying mechanism of American Ginseng Polysaccharide(AGP)against H_(2)O_(2)-induced oxidative damage in HepG2 cells were investigated based on the Nuclear Factor-erythroid 2-related Factor 2(Nrf2)/Heme Oxygenase-1(HO-1)signaling pathway.H_(2)O_(2)at a concentration of 300μmol/L was selected for establishing an oxidative damage model of HepG2 cells(cell survival rate:62.23%),and 320,640 or 1280μg/mL of AGP was given for intervention,and the reactive oxygen species(ROS),superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH),and malondialdehyde(MDA)levels in each group of cells were measured separately;the expressions of Nrf2,SOD1,SOD2,HO-1,and CAT proteins in the cells were analyzed by Western blot.The results showed that the AGP treatment had insignificant effect on the proliferation of HepG2 cells,and AGP at the three dose could significantly increase the survival rate of oxidatively damaged HepG2 cells,which were 89.36%,91.42%,and 91.06%,respectively;The levels of ROS(15.74%,20.71%,and 25.39%)and MDA content in oxidatively damaged cells were significantly reduced,and the levels of intracellular SOD,GSH,and CAT proteins were effectively elevated;Western blot results showed that AGP could significantly up-regulate the protein expression levels of Nrf2,SOD1,SOD2,HO-1,and CAT(P<0.05,P<0.01).In summary,AGP can ameliorate the oxidative damage in HepG2 cells by regulating the Nrf2/HO-1 signaling pathway.The results of this study can provide more data support for the further development and utilization of AGP.
作者
李健豪
宋莹莹
杜跃中
郭畅冰
王国明
徐芳菲
LI Jianhao;SONG Yingying;DU Yuezhong;GUO Changbing;WANG Guoming;XU Fangfei(Jilin Ginseng Research Institute,Changchun 130031,China)
出处
《现代食品科技》
北大核心
2025年第8期40-50,共11页
Modern Food Science and Technology
基金
吉林省中医药科技项目(2023085)
吉林省部门预算科学项目(CZ2403002)。
关键词
西洋参多糖
HEPG2细胞
氧化损伤
NRF2
american ginseng polysaccharide
HepG2 cells
oxidative damage
Nrf2(Nuclear factor-erythroid 2-related factor 2)
