摘要
目的:评估燕窝阿胶复方对小鼠睡眠改善的作用及其作用机制。方法:将Balb-c小鼠随机分为五组,分别为空白对照组、阳性药物组、燕窝阿胶复方低剂量组(5 g/kg)、中剂量组(10 g/kg)和高剂量组(20 g/kg)。每组小鼠每日给药一次,持续给药30 d。通过直接睡眠实验、戊巴比妥钠阈下剂量催眠实验、戊巴比妥钠睡眠时间延长实验以及巴比妥钠睡眠潜伏期实验评估燕窝阿胶复方对小鼠睡眠的改善效果。同时,运用网络药理学方法初步探讨燕窝阿胶复方改善小鼠睡眠的潜在作用机制。为了验证网络药理学的解析结果,本研究采用酶联免疫分析试剂盒比色法,测定小鼠脑组织中血清素5-羟色胺(5-hydroxytryptamine,5-HT)、多巴胺(dopamine,DA)、色氨酸羟化酶(tryptophan hydroxylase,TPH)以及γ-氨基丁酸(γ-aminobutyric acid,GABA)的水平。结果:燕窝阿胶复方并不能诱导小鼠直接进入睡眠状态,而是通过改善小鼠睡眠质量来达到改善睡眠的效果。与空白对照组相比,燕窝阿胶复方低、中、高剂量组分别使小鼠睡眠率提高了50%、70%和80%;三个剂量组均显著延长了小鼠戊巴比妥钠诱导的睡眠时间(P<0.01);中、高剂量组显著缩短了小鼠的睡眠潜伏期(P<0.05或P<0.01)。网络药理学分析揭示,燕窝阿胶复方主要通过调节5-HT、GABA、DA等神经递质的突触通路来改善睡眠。实验数据进一步证实,与空白对照组相比,燕窝阿胶复方中、高剂量组能显著增加小鼠脑组织中TPH含量(P<0.01或P<0.05);三个剂量组均显著提升了小鼠脑组织中5-HT和GABA的含量(P<0.01或P<0.05)。另外,相较于空白对照组,燕窝阿胶复方的三个剂量组均显著降低了小鼠脑组织中DA的含量(P<0.01)。结论:燕窝阿胶复方对小鼠睡眠具有显著的改善作用,其作用机制主要涉及调节5-HT、GABA、DA等神经递质的突触通路。
Objective:To evaluate improvement of sleep function and mechanism of Yanwo Ejiao compound in mice.Methods:Balb-c mice were randomly assigned to five groups:A blank control group,a positive drug group,and three Yanwo Ejiao compound groups receiving low(5 g/kg),medium(10 g/kg),and high(20 g/kg)doses.The compound was administered once daily for 30 consecutive days.Sleep improvement was assessed by direct sleep test,subthreshold dose hypnosis test with pentobarbital sodium,pentobarbital sodium-induced sleep time extension test,and barbital sodium sleep latency test.Additionally,network pharmacology methods were employed to preliminarily investigate the compound's potential mechanism.To validate the findings,enzyme-linked immunosorbent assay kits were used to measure brain levels of 5-hydroxytryptamine(5-HT),dopamine(DA),tryptophan hydroxylase(TPH),andγ-aminobutyric acid(GABA).Results:Yanwo Ejiao compound did not induce mice directly into a hypnotic state,but significantly improved sleep quality in mice.Compared to the blank control group,the low,medium,and high-dose groups increased the sleep rate by 50%,70%,and 80%,respectively.Three Yanwo Ejiao compound groups significantly prolonged pentobarbital sodium-induced sleep time(P<0.01).The medium and high-dose groups also significantly reduced sleep latency(P<0.05 or P<0.01).Network pharmacology analysis suggested that the compound improved sleep by modulating neurotransmitter synaptic pathways,including those of 5-HT,GABA,and DA.Experimental data confirmed that,compared to the control group,the medium and high-dose group significantly increased TPH levels(P<0.01 or P<0.05)and three compound groups elevated 5-HT and GABA levels in brain tissue(P<0.01 or P<0.05).Besides,three Yanwo Ejiao compound groups significantly decreased DA content(P<0.01).Conclusion:Yanwo Ejiao compound exerts a notable sleep-improvement effect in mice,primarily through the regulation of synaptic pathways involving neurotransmitters such as 5-HT,GABA,and DA.
作者
郑海云
王志钢
王少南
王斌
ZHENG Haiyun;WANG Zhigang;WANG Shaonan;WANG Bin(Science and Technology Collaborating Center for Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;China Science and Technology Department of National Administration of Traditional Chinese Medicine,Beijing 100027,China)
出处
《食品工业科技》
北大核心
2025年第19期388-397,共10页
Science and Technology of Food Industry
基金
国家自然科学基金(82274685)。
关键词
燕窝阿胶复方
改善睡眠
作用机制
网络药理学
Yanwo Ejiao compound
sleep improvement
mechanism
network pharmacology
