摘要
采用非靶向代谢组学方法,分析锦灯笼宿萼、果、茎和根等不同部位间代谢物的差异性。运用超高效液相色谱-四极杆-静电场轨道肼高分辨质谱(UHPLC-Q-Orbitrap HRMS),通过主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)、聚类分析(HCA)、代谢物及其代谢通路差异性分析,对锦灯笼不同部位的代谢组学特征进行研究。结果显示,从锦灯笼不同部位中共检测到1416种代谢物,其含量组成以有机酸、生物碱、氨基酸及其衍生物等为主;锦灯笼不同部位的代谢物存在显著差异,宿萼中代谢物种类含量较高的是黄酮、苯丙素、维生素类,果中代谢物种类含量较高的是有机酸、生物碱、甾体类,茎中代谢物种类含量较高的是萜、黄酮、酯、酚酸类,根中代谢物种类含量较高的是糖、脂、氨基酸及其衍生物类;OPLS-DA分析筛选出113个差异代谢物(变量权重值VIP>1.7和P<0.05),其中有机酸、生物碱、脂类等成分占比较高,主要参与不饱和脂肪酸生物合成、柠檬酸循环、丙酮酸等代谢途径;在鉴定出的黄酮类成分中有20%均为差异代谢物,并且此类成分在差异代谢物中变异比例最大。该研究可为锦灯笼不同部位的成分差异以及资源的综合开发利用提供理论参考。
Non-targeted metabolomics techniques were used to analyze the metabolite differences in the calyx,fruit,stem,and root of Physalis alkekengi L.var.franchetii(PAVF).The metabolomic profiles of PAVF were investigated by UHPLC-Q-Orbitrap HRMS with PCA,OPLS-DA,HCA,and metabolite and metabolic pathway difference analysis.The results showed that 1416 metabolites were detected,with the highest proportion of organic acids,alkaloids,and amino acids and their derivatives.The results of multivariate statistical analysis showed that there were significant differences amongst different parts of PAVF.The calyx contained high flavonoids,phenylpropanoids,and vitamins.The fruit contained high organic acids,alkaloids,and steroids.The stem contained high terpenes,flavonoids,esters,and phenolic acids.The root contained high sugars,lipids,and amino acids and their derivatives.OPLS-DA analysis identified 113 differential metabolites(VIP>1.7 and P<0.05),with the highest proportion of organic acids,alkaloids,and lipids,which were mainly involved in the metabolic pathways of unsaturated fatty acid biosynthesis,citrate cycle,and pyruvate metabolism.Twenty percent of the flavonoids identified were differential metabolites,and such components had the largest proportion of variation among differential metabolites.This study may provide a theoretical reference for the composition differences and efficient resource utilization of PAVF.
作者
金银萍
李政
邓莉
张浩
JIN Yinping;LI Zheng;DENG Li;ZHANG Hao(Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112,China;Jilin Agricultural Science and Technology University,Jilin 132101,China;Jilin Province Qingmei Agricultural Technology Co.,Ltd.,Tonghua 134100,China)
出处
《食品工业科技》
北大核心
2025年第19期287-296,共10页
Science and Technology of Food Industry
基金
中国农业科学院科技创新计划项目(CAAS-ASTIP-2021-ISAPS)
吉林省创新平台和人才专项项目(20220505006ZP)。
关键词
锦灯笼
不同部位
非靶向代谢组学
差异代谢物
Physalis alkekengi L.var.franchetii
different parts
non-targeted metabolomics
differential metabolites
