摘要
基于网络药理学和动物实验,探讨中药民族药柯孜木克(Kezmekh,KZMK)颗粒对慢性细菌性前列腺炎(chronic bacterial prostatitis,CBP)的防治作用。运用网络药理学方法预测KZMK颗粒作用于CBP的核心靶点及信号通路,共筛选出181个潜在作用靶点,主要涉及4条关键信号通路,通过KEGG富集分析确定磷脂酰肌醇3激酶-蛋白激酶B(phosphatidylinositol 3-kinase-protein kinase B,PI3K-AKT)信号通路与CBP的关联最为显著。为验证预测结果,成功建立了大肠杆菌(Escherichia coli)诱导的大鼠CBP模型,通过苏木精-伊红染色(hematoxylin and eosin staining,HE)观察发现,KZMK能显著改善前列腺组织病理形态,减少炎症细胞浸润;酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)显示,KZMK可显著降低血清中白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平(P<0.05或P<0.01)。蛋白质免疫印迹(Western blot)分析证实,KZMK能显著下调前列腺组织中PI3K、AKT以及TNF-α、IL-1β和IL-6蛋白的表达(P<0.001或P<0.01)。综合研究结果表明,KZMK可能通过抑制促炎因子IL-6和TNF-α的释放,调控PI3K-AKT信号通路关键蛋白的磷酸化表达,从而减轻前列腺组织炎症反应,发挥对CBP的治疗作用。
Based on network pharmacology and animal experiments,this study investigated the preventive and thera-peutic effects of Kezmekh(KZMK)granules,a traditional Chinese medicine and ethnic medicine,on chronic bacterial prostatitis(CBP).Network pharmacology methods were used to predict the core targets and signaling pathways of KZMK granules acting on CBP,and a total of 181 potential targets were screened,primarily involving four key signaling pathways,with KEGG enrichment analysis confirming the phosphatidylinositol 3-kinase-protein kinase B(PI3K-AKT)sig-naling pathway as the most significantly associated with CBP.To validate these predictions,the study successfully estab-lished a rat CBP model induced by Escherichia coli.Hematoxylin and eosin(HE)staining revealed that KZMK signifi-cantly improved prostate tissue histopathology and reduced inflammatory cell infiltration.Enzyme-linked immunosorbent assay(ELISA)demonstrated that KZMK markedly decreased serum levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)(P<0.05 or P<0.01).Western blot analysis confirmed that KZMK significantly downregulated the expression of PI3K,AKT,as well as TNF-α,IL-1β,and IL-6 proteins in prostate tissues(P<O.001 or P<0.O1).In summary,these findings suggest that KZMK may exert its therapeutic effects on CBP by inhibiting the release of pro-inflammatory factors IL-6 and TNF-α,regulating the phosphorylation of key proteins in the PI3K-AKT signaling pathway,thereby alleviating inflammatory responses in prostate tissues.
作者
努尔巴合提·候瓦提
胡梦颖
叶力夏提·达那别克
程波
徐磊
哈木拉提·哈斯木
Nurbahti-Huwati;Hu Mengying;Yelishat·Danabek;Cheng Bo;Xu Lei;Hamurat·Hasmu(Xinjiang Uygur Autonomous Region Institute of Pharmacology,Urumqi 830034,Xinjiang,China)
出处
《生物资源》
2025年第4期379-388,共10页
Biotic Resources
基金
自治区公益性科研院所基本科研业务费项目(KY2021099)
自治区卫生健康青年医学科技人才专项科研项目(WJWY-202224)。
