摘要
采后果实抗坏血酸(ascorbic acid,AsA)包含氧化态和还原态2种存在形式,贮藏过程中AsA含量的下降是影响果实品质的重要因素。为探究2,4-表油菜素内酯(2,4-epibrassinolide,EBR)对采后草莓AsA代谢的调控机制,研究采用5μmol/L EBR对采后草莓果实进行喷洒处理,并在(20±1)℃、90%~95%相对湿度条件下进行为期5 d的贮藏实验。结果表明,EBR处理显著抑制了还原态AsA、氧化态AsA[脱氢抗坏血酸(dehydroascorbic acid,DHA)]及总AsA(AsA+DHA)含量的下降,同时维持了AsA/DHA比值的平衡。通过转录组学分析,发现EBR处理共调控了3309个差异表达基因,其中1491个基因上调,1818个基因下调。进一步结合实时荧光定量PCR(quantitative real-time polymerase chain reaction,qRT-PCR)技术,明确了EBR处理后草莓果实AsA合成代谢相关基因的转录变化,发现FaMIOX和FaAPX基因的上调表达对AsA/DHA平衡具有积极作用,且其表达可能受WRKY家族转录因子的调控。研究揭示了EBR通过调控关键基因表达延缓采后草莓AsA含量下降的分子机制,为EBR在采后果实保鲜中的应用提供了理论依据和实践参考。
Postharvest fruit ascorbic acid(AsA)exists in both oxidized and reduced forms and the decline of AsA content during postharvest storage is a critical factor affecting fruit quality.To investigate the regulatory mechanism of 2,4-epibrassinolide(EBR)on AsA metabolism in postharvest strawberries,this study applied 5μmol/L of EBR to strawberry fruit and conducted a 5-day storage experiment at(20±1)℃with 90%-95%relative humidity.Results showed that EBR treatment significantly inhibited the decline in reduced AsA,dehydroascorbic acid(DHA)and total AsA contents,while maintaining the AsA/DHA ratio.Transcriptome analysis revealed that EBR treatment regulated 3309 differentially expressed genes,including 1491 upregulated and 1818 downregulated genes.Further analysis using quantitative real-time PCR(qRT-PCR)clarified the transcriptional changes in AsA metabolic pathway-related genes in EBR-treated strawberry fruits.Notably,the upregulated expression of FaMIOX and FaAPX genes positively contributed to AsA/DHA homeostasis,potentially regulated by WRKY family transcription factors.This study elucidates the molecular mechanism by which EBR delays AsA degradation in postharvest strawberries through the regulation of key gene expression,providing a theoretical basis and practical reference for the application of EBR in postharvest fruit preservation.
作者
刘玲娜
罗灵灵
左小霞
周倩
周鑫
纪淑娟
李美琳
LIU Lingna;LUO Lingling;ZUO Xiaoxia;ZHOU Qian;ZHOU Xin;JI Shujuan;LI Meilin(College of Food Science,Shenyang Agricultural University,Shenyang 110866,China;College of Food Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《食品与发酵工业》
北大核心
2025年第17期205-213,共9页
Food and Fermentation Industries
基金
辽宁省教育厅基本科研项目(JYTYB2024018)。
关键词
2
4-表油菜素内酯
草莓
抗坏血酸
转录组
差异表达基因
2,4-epibrassinolide
strawberry
ascorbic acid
transcriptome
differentially expressed genes
