摘要
为了明确绣球花叶枯病的病原菌,本研究通过组织分离法对采集自江苏省南京市的绣球品种无尽夏叶片发病组织进行分离,经回接试验确定菌株YK-B是致病菌,研究发现,YK-B菌株接种至健康绣球花叶片上能引起和采集的绣球花叶片发病组织一致的叶枯病症状。通过形态学观察并结合多基因联合系统发育分析,病原菌菌株YK-B被鉴定为链格孢菌(Alternaria alternata);进一步进行病原菌菌株YK-B在不同碳源、氮源、温度和pH值条件下的菌丝生长试验,结果表明,YK-B菌丝生长最适碳源是棉子糖,最适氮源是蛋白胨,最适温度是25℃,适宜的pH值为6~8。通过测定7种杀菌剂对致病菌菌株YK-B的室内毒力,结果表明杀菌剂氢氧化铜的抑菌效果最好。本研究结果对绣球花叶枯病的诊断和防治具有一定的指导意义。
To identify the pathogen responsible for hydrangea leaf blight,a tissue isolation method was employed on diseased leaf samples of Hydrangea macrophylla Endless Summer collected from Nanjing City,Jiangsu Province,China.Through back-inoculation experiments,strain YK-B was identified as the pathogenic fungus.The study found that inoculation of healthy hydrangea leaf tissues with strain YK-B induced leaf blight symptoms consistent with those observed on the originally collected diseased samples.Based on morphological characteristics and multigene phylogenetic analysis,the pathogenic strain YK-B was identified as Alternaria alternata.Furthermore,mycelial growth experiments were conducted with the pathogenic strain YK-B under varying conditions of carbon sources,nitrogen sources,temperature,and pH.The results indicated that the optimal conditions for mycelial growth of YK-B were raffinose as the carbon source,peptone as the nitrogen source,a temperature of 25℃,and a pH range of 6 to 8.The results of in vitro toxicity assays of seven fungicides against the pathogenic strain YK-B revealed that copper hydroxide exhibited the most effective inhibition.The findings of this study provide practical guidance for the diagnosis and management of hydrangea leaf blight.
作者
陈慧杰
毛俊丹
邓喆
刘欣童
陈双双
冯景
金玉妍
邓衍明
CHEN Huijie;MAO Jundan;DENG Zhe;LIU Xintong;CHEN Shuangshuang;FENG Jing;JIN Yuyan;DENG Yanming(Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;School of Civil Engineering and Architecture,Anhui University of Technology,Ma’anshan 243032,China;College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211816,China)
出处
《江苏农业学报》
北大核心
2025年第8期1499-1507,共9页
Jiangsu Journal of Agricultural Sciences
基金
江苏省农业科技自主创新基金项目[CX(2023)3062]
华东地区花卉生物学国家林业和草原局重点实验室开放基金项目(KFE202401)
中央财政林业科技推广示范项目[苏(2024)TG03]。
关键词
绣球花
叶枯病
病原菌鉴定
生物学特性
毒力测定
Hydrangea macrophylla
leaf blight
pathogen identification
biological characteristics
toxicity test
