摘要
粪便微生物群移植(FMT)作为一项新兴技术,已被证实能够有效重塑家禽肠道微生物组成。本试验旨在探究FMT早期干预对雏鸭回肠微生物组成的影响。试验选择120只1日龄的健康三穗鸭,随机分为2个组,移植组(FMT)和对照组(CON),每组6个重复,每个重复10只,试验前3 d,CON组饲喂基础日粮,FMT组灌喂500μL粪菌液,试验期42 d,通过16S rRNA基因V3~V4区测序,分析三穗鸭回肠Alpha和Beta多样性及细菌组成的变化。结果表明:在门水平上,移植组的厚壁菌门、梭杆菌门丰度较对照组分别提高了27.45(P>0.05)和10.53个百分点(P<0.01);变形菌门、放线菌门丰度分别降低了30.81(P>0.05)和6.35个百分点(P>0.05)。在属水平上,移植组乳酸杆菌的丰度较对照组提高了6.72个百分点(P>0.05);幽门螺杆菌、肠球菌和葡萄球菌的丰度分别降低了27.21(P<0.05)、1.95(P>0.05)和3.19个百分点(P>0.05)。此外,FMT显著提高了回肠细菌的Alpha多样性。综上表明,FMT能够重塑供体特异性的微生物群落,可能是改善家禽肠道健康的潜在策略。
Fecal microbiota transplantation(FMT)is an emerging technology,has been shown to reshape the gut microbiome of poultry effectively.This experiment aimed to investigate the early intervention effects of FMT on the ileal microbiome composition in ducks.A total of 120 healthy one-day-old Sansui ducks were selected and randomly divided into two groups:the FMT group(FMT)and the control group(CON),with 6 replicates per group and 10 ducks per replicate.For the first 3 days of the experiment,the CON group were fed a basal diet,while the FMT group were orally administrated with 500μL fecal microbiota suspension.The experiment lasted for 42 days.Alpha and Beta diversity of the ileal microbiome and the bacterial composition were analyzed through 16S rRNA gene sequencing of the V3~V4 region.The results demonstrated that at the phylum level,the abundance of Firmicutes and Bacteroides in the FMT group increased by 27.45 percentage points(P>0.05)and 10.53 percentage points(P<0.01),respectively,compared with the control group.Meanwhile,the abundance of Proteobacteria and Actinobacteria decreased by 30.81 percentage points(P>0.05)and 6.35 percentage points(P>0.05),respectively.At the genus level,Lactobacillus in the FMT group increased by 6.72 percentage points(P>0.05)compared with the control group.Moreover,the abundance of Helicobacter,Enterococcus,and Staphylococcus decreased by 27.21 percentage points(P<0.05),1.95 percentage points(P>0.05),and 3.19 percentage points(P>0.05),respectively.Notably,FMT significantly altered the Alpha diversity of the ileal microbiome.Taken together,FMT could reshape the donor-specific microbial community and may serve as a potential strategy for improving gut health in poultry.
作者
岳雍
何志强
祝永才
姚炳浓
廖富友
杨胜林
YUE Yong;HE Zhiqiang;ZHU Yongcai;YAO Bingnong;LIAO Fuyou;YANG Shenglin(Key Laboratory of Animal Genetics,Breeding and Reproduction in the plateau Mountainous Rejion,Ministry of Eduction,Guizhou University,Guiyang,Guizhou Province 550025,China;Key Laboratory of Animal Genetics,Breeding and Reproduction of Guizhou province,Guizhou University,Guiyang,Guizhou Province 550025,China;College of Animal Science,Guizhou University,Guiyang,Guizhou Province 550025,China;Agricultural service center of Dongdi township,Nayong county,Bijie,Guizhou Province 553304,China)
出处
《中国饲料》
北大核心
2025年第17期46-52,共7页
China Feed
基金
国家自然科学基金(31960682)
贵州省科技厅平台项目(黔科合平台人才[2019]5203)
中央引导地方科技发展专项资金项目(黔科中引地[2019]4021)
贵州省成果转化项目(黔科合成果[2020]1Y41)
贵州省第六批人才基地项目(黔人颁发[2018]3号)
黔科合支撑(2019)2283号。
