摘要
目的检测猫眼综合征染色体候选基因2(CECR2)在透明细胞肾细胞癌(ccRCC)中的表达情况,探讨CECR2表达与肿瘤相关巨噬细胞(TAMs)的关系及其临床病理意义。方法利用TCGA数据库分析ccRCC的基因差异,明确CECR2是否为差异性基因;采用免疫组织化学染色法检测本院112例ccRCC组织、88例癌旁正常上皮组织中CECR2和TAMs的表达情况,并结合临床病理资料,应用统计学方法分析其相关性。结果基于TCGA数据库的差异基因分析提示CECR2为显著下调基因。免疫组织化学染色显示CECR2在ccRCC组织中表达下调,癌组织中的阳性率(11.61%)显著低于癌旁正常上皮组织(96.69%);CECR2表达下调与ccRCC的WHO/ISUP核分级、T分期及肿瘤大小密切相关。ccRCC组织中M1型巨噬细胞(CD86阳性细胞)的平均浸润密度显著低于癌旁正常上皮组织(4.36个/HPF vs.8.99个/HPF),M2型巨噬细胞(CD206阳性细胞)的平均浸润密度显著高于癌旁正常上皮组织(16.72个/HPF vs.4.83个/HPF),显示ccRCC中浸润的巨噬细胞以M2型为主。进一步分析ccRCC组织中CECR2表达与M2型巨噬细胞分布的关系,显示CECR2表达与M2型巨噬细胞(CD206阳性细胞)的浸润密度呈显著负相关,而与M1型巨噬细胞(CD86阳性细胞)的浸润密度呈正相关。结论ccRCC组织中存在CECR2表达水平的显著下调,且其表达下调与肿瘤相关巨噬细胞的分布密切有关,CECR2可能通过诱导M2型巨噬细胞极化,进而促进ccRCC的进展,CECR2有望会成为ccRCC的一个新的潜在治疗靶点。
Objective We aimed to detect the expression of cat eye syndrome chromosome region candidate 2(CECR2)in clear cell renal cell carcinoma(CCRCC),and elucidate the correlation between CECR2 expression and tumor-associated macrophages(TAMs)and its clinicopathological significance.Methods We utilized the TCGA database to analyze the differentially expressed genes(DEGs)in ccRCC,identifying CECR2 as a DEG.Immunohistochemical staining was performed on 112 ccRCC samples and 88 adjacent normal epithelial tissues from the Nanchong Central Hospital to assess CECR2 and TAMs expressions.Statistical methods analyzed the correlation between CECR2,TAMs,and the clinicopathological features of ccRCC.Results Differential analysis based on TCGA database identified that CECR2 was a significantly down-regulated gene.Immunohistochemical staining showed that CECR2 was notably downregulated in ccRCC tissues,with a significantly lower positive rate(11.61%)compared to adjacent normal epithelial tissues(96.69%).Its expression was also significantly associated with WHO/ISUP nuclear grade、T stage,and maximum tumor diameter.Furthermore,the mean infiltration density of M1 macrophages(CD86-positive cells)was significantly lower in ccRCC tissues than in adjacent normal epithelial tissues(4.36/HPF vs 8.99/HPF),whereas M2 macrophages(CD206-positive cells)was significantly higher in ccRCC tissues than in adjacent normal epithelial tissues(16.72/HPF vs 4.83/HPF).The infiltration of macrophages in ccRCC was mainly M2 type.Further analysis indicated a significant negative correlation between CECR2 and M2 macrophages(CD206-positive cells)expression,and a significant positive correlation between CECR2 and M1 macrophages(CD86-positive cells)expression in ccRCC tissues.Conclusion CECR2 was significantly down-regulated in ccRCC tissues,and its expression correlated with the distribution of tumor-associated macrophages.CECR2 may influence ccRCC progression by inducing the polarization of M2 macrophages.CECR2 is expected to be a new potential therapeutic target for ccRCC.
作者
彭丽娟
伍季
张旭乾
蒋卓颖
魏钰秦
龙琼先
PENG Lijuan;WU Ji;ZHANG Xuqian;JIANG Zhuoyin;WEI Yuqin;LONG Qiongxian(Department of Pathology,The Affiliated Nanchong Central Hospital of North Sichuan Medical College,Beijing Anzhen Nanchong Hospital of Capital Medical University,Nanchong 637000,Sichuan,China;Department of Urology,The Affiliated Nanchong Central Hospital of North Sichuan Medical College,Beijing Anzhen Nanchong Hospital of Capital Medical University,Nanchong 637000,Sichuan,China)
出处
《西部医学》
2025年第9期1286-1292,共7页
Medical Journal of West China
基金
四川省卫生健康委员会课题项目(20PJ297)
南充市科技局项目(KY-22JCYJPT0033)。
