摘要
目的比较糖尿病患者和健康人改良富血小板纤维蛋白(A-PRF)中关键成骨生长因子与炎症因子的释放差异。方法按纳排标准分为糖尿病组和健康人组(每组n=8),分别采集静脉血,离心制备A-PRF膜并孵育后,在15 min、60 min、8 h、1 d、3 d、10 d、14 d和21 d收集析出液,通过酶联免疫吸附实验检测血小板衍生生长因子-BB(PDGF-BB)、血管内皮生长因子(VEGF)、转化生长因子-β(TGF-β)、表皮生长因子(EGF)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的释放量并进行比较分析。结果(1)糖尿病组A-PRF中PDGF-BB累积释放量始终低于健康人组,且从15 min开始2组差异具有统计学意义(P<0.01);(2)糖尿病组A-PRF中VEGF累积释放量在1 d至21 d均低于健康人组(P<0.01);(3)2组A-PRF中的EGF累积释放量差异无统计学意义(P>0.05);(4)糖尿病组A-PRF中TGF-β累积释放量从1 d开始始终低于健康人组,且差异具有统计学意义(P<0.05);(5)糖尿病组A-PRF中TNF-α累积释放量从8 h开始始终都高于健康人组(P<0.05);(6)2组A-PRF中IL-6累积释放量差异无统计学意义(P>0.05)。结论与健康人相比,糖尿病患者A-PRF中成骨关键生长因子PDGF-BB、VEGF、TGF-β累积释放量显著降低,炎症因子TNF-α则持续升高,而EGF与IL-6的总体释放量未见显著差异。
Objective To compare the release kinetics of key osteogenic growth factors and inflammatory cytokines in advanced platelet-rich fibrin(A-PRF)between patients with diabetes mellitus(DM)and healthy populations.Methods Participants meeting the inclusion and exclusion criteria were assigned to either the DM group(n=8)or the healthy control group(n=8).Venous blood was centrifuged to prepare A-PRF membranes,and eluates were collected at 15 min,60 min,8 h,1 d,3 d,10 d,14 d,and 21 d post-incubation.The concentrations of platelet-derived growth factor-BB(PDGF-BB),vascular endothelial growth factor(VEGF),transforming growth factor-beta(TGF-β),epidermal growth factor(EGF),tumor necrosis factor-alpha(TNF-α),and interleukin-6(IL-6)were quantified using enzyme-linked immunosorbent assay(ELISA)and analyzed comparatively.Results(1)Cumulative PDGF-BB release from A-PRF in the DM group was consistently lower than that in healthy controls,with statistically significant differences observed from 15 min onward(P<0.01).(2)VEGF release from A-PRF was significantly reduced in the DM group from day 1 to day 21 compared to controls(P<0.01).(3)No statistically significant difference in cumulative EGF release was observed between the two groups(P>0.05).(4)Cumulative TGF-βrelease from A-PRF was significantly lower in the DM group from day 1 onward(P<0.05).(5)TNF-αrelease from A-PRF was significantly higher in the DM group than in healthy controls from 8 h onward(P<0.05).(6)No statistically significant difference in cumulative IL-6 release was observed between the two groups(P>0.05).Conclusion Compared to healthy individuals,DM patients exhibited significantly lower cumulative release of osteogenic growth factors(PDGF-BB,VEGF,TGF-β)from A-PRF and elevated levels of the inflammatory cytokine TNF-α.No significant differences were observed in EGF and IL-6 release.
作者
农英娇
黎永薇
陆婵
邓翰林
唐佳勤
李淑洁
梁丽华
Nong Yingjiao;Li Yongwei;Lu Chan;Deng Hanlin;Tang Jiaqin;Li Shujie;Liang Lihua(Department of Dental Implant,Affiliated Stomatology Hospital of Guilin Medical University,Guilin 541004,Guangxi,China)
出处
《中国口腔种植学杂志》
2025年第4期370-376,共7页
Chinese Journal of Oral Implantology
基金
2023年广西高校中青年教师科研基础能力提升项目(2023KY0503)。
关键词
改良富血小板纤维蛋白
糖尿病
生长因子
炎症因子
骨愈合
Advanced platelet-rich fibrin(A-PRF)
Diabetes mellitus
Growth factors
Inflammatory factors
Bone healing
