摘要
以白芷为原料,采用冷水反复冻融浸提、乙醇沉淀制备白芷多糖(Angelica dahurica polysaccharide,ADP)。通过单因素试验研究料液比、冷冻时间、冻融次数、醇沉时间、醇沉体积分数对白芷多糖得率的影响,再利用正交试验得到最优提取工艺:料液比1:30(g/mL)、冷冻时间8 h、冻融3次、醇沉时间12 h、醇沉体积分数80%。利用Sevag法、H_(2)O_(2)处理和透析袋过滤,获得精制白芷多糖;再利用柱层析法进一步纯化,得到纯化白芷多糖(ADP-1)。采用紫外可见分光光度法、傅里叶变换红外光谱、高效液相色谱仪以及扫描电子显微镜解析ADP-1一级结构,得到ADP-1多糖含量为90.1%、平均分子质量为12.02×10^(3)u。同时,ADP-1溶液呈现非牛顿流体中假塑性流体的剪切稀化特性,表观黏度对温度、p H值及质量分数变化较为敏感,属于典型的弱凝胶。
Angelica dahurica polysaccharide(ADP)was prepared by freezing and thawing extraction with cold water and ethanol precipitation.The effects of solid-liquid ratio,freezing time,freeze-thaw times,alcohol precipitation time and alcohol precipitation concentration on polysaccharide yield of Angelica dahurica were studied by single factor test.The optimal extraction process was obtained by orthogonal test:solid-liquid ratio 1∶30(g/mL),freezing time 8 h,freezing and thawing times 3 times,alcohol precipitation time 12 h and alcohol precipitation concentration 80%.The refined Angelica dahurica polysaccharides were obtained by Sevag method,H_(2)O_(2) treatment and dialysis filtration.The purified Angelica dahurica polysaccharide(ADP-1)was further purified by column chromatography.The primary structure of ADP-1 was analyzed by UV-visible spectrophotometry,Fourier transform infrared(FTIR)spectroscopy,high performance liquid chromatography(HPLC)and scanning electron microscope.The polysaccharide content of ADP-1 was 90.1%with an average molecular weight of 12.02×10^(3) u.At the same time,ADP-1 solution showed the shear thinning characteristics of pseudo-plastic fluid in non-Newtonian fluids,and the apparent viscosity was sensitive to temperature,pH and mass concentration changes,which was a typical weak gel.
作者
涂剑秋
曹珍
夏南
王怡
王清
李建芳
TU Jianqiu;CAO Zhen;XIA Nan;WANG Yi;WANG Qing;LI Jianfang(College of Food Science and Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,China)
出处
《食品科技》
北大核心
2025年第7期179-187,共9页
Food Science and Technology
基金
河南省高等学校重点科研项目(24B550016,22B550016,23B550006)
河南省科技攻关项目(232102230071)
信阳农林学院科技创新团队项目(XNKJTD-002)。
关键词
白芷多糖
提取工艺优化
分离纯化
结构表征
流变学特性
Angelica dahurica polysaccharide
extraction process optimization
separation and purification
structural characterization
rheological properties
