摘要
目的探讨非小细胞肺癌(NSCLC)组织中磷蛋白1(SPP1)、转移抑制因子1(MTSS1)、溶质载体家族7成员11(SLC7A11)表达与患者临床病理特征及预后的关系。方法选取2019年7月—2021年6月黄冈市中心医院肿瘤内科收治的NSCLC手术患者204例为研究对象。免疫组化法检测患者癌组织及癌旁组织中SPP1、MTSS1、SLC7A11表达;根据术后3年内死亡情况分为死亡组(n=50)及生存组(n=143);多因素Cox回归分析NSCLC预后的影响因素;Kaplan-Meier曲线分析SPP1、MTSS1、SLC7A11表达与NSCLC预后的关系。结果癌组织中SPP1、SLC7A11阳性表达率均显著高于癌旁组织,MTSS1阳性表达率明显低于癌旁组织(χ^(2)/P=90.362/<0.001、100.885/<0.001、102.073/<0.001);NSCLC患者癌组织SPP1阳性表达和SLC7A11阳性表达中,肿瘤直径≥5 cm、TNM分期为Ⅲ期、有淋巴结转移、有吸烟史的患者占比更高(SPP1:χ^(2)/P=5.408/0.020、10.636/<0.001、6.838/<0.001、10.352/<0.001;SLC7A11:χ^(2)/P=6.965/<0.001、7.638/<0.001、10.597/<0.001、16.657/<0.001),而在MTSS1阳性表达中,TNM分期Ⅰ~Ⅱ期、无淋巴结转移、无吸烟史的患者占比更高(χ^(2)=9.681、10.611、7.187,P均<0.001);与生存组比较,死亡组有吸烟史、有职业暴露、TNM为Ⅲ期、淋巴结转移者占比明显较高(χ^(2)/P=7.501/<0.001、4.205/0.040、12.861/<0.001、10.907/<0.001);死亡组患者SPP1、SLC7A11阳性率高于生存组,MTSS1阳性率低于生存组(χ^(2)/P=11.273/<0.001、11.490/<0.001、7.603/0.006);多因素Cox回归结果显示,TNMⅢ期、有淋巴结转移、SPP1阳性、SLC7A11阳性、有吸烟史均为NSCLC预后不良的危险因素,MTSS1阳性为NSCLC预后不良的保护因素[HR(95%CI)=2.369(1.623~3.458)、1.843(1.308~2.597)、2.014(1.396~2.906)、1.986(1.420~2.777)、2.259(1.370~3.724)、0.856(0.754~0.972)];SPP1、SLC7A11阳性表达的NSCLC患者3年总生存率分别低于SPP1、SLC7A11阴性表达(Log Rankχ^(2)/P=9.988/0.002、10.608/0.001),MTSS1阳性表达患者3年总生存率高于MTSS1阴性表达(Log Rankχ^(2)/P=7.884/0.005)。结论NSCLC患者癌组织中SPP1、SLC7A11阳性表达率高,MTSS1阳性表达率低,且三者表达情况与NSCLC病理特征及预后密切相关。
Objective To investigate the relationship between the expression of secreted phosphoprotein 1(SPP1),metastasis suppressor 1(MTSS1),and solute carrier family 7 member 11(SLC7A11)in non-small cell lung cancer(NSCLC)tissues and the clinical pathological characteristics and prognosis of patients.Methods A total of 204 NSCLC patients admitted to the Department of Oncology of Huanggang Central Hospital from July 2019 to June 2021 were selected as the study objects.The expression of SPP1,MTSS1 and SLC7A11 in cancer tissues and adjacent tissues were detected by immunohistochemistry.According to the death situation within 3 years after operation,the patients were divided into death group(n=50)and survival group(n=143).Multivariate Cox regression was used to analyze the prognostic factors of NSCLC.Kaplan-Meier curve was used to analyze the relationship between the expression of SPP1,MTSS1 and SLC7A11 and the prognosis of NSCLC.Results The positive expression rates of SPP1 and SLC7A11 in cancer tissues were significantly higher than those in paracancer tissues,and the positive expression rates of MTSS1 were significantly lower than that in paracancer tissues(χ^(2)/P=90.362/<0.001,100.885/<0.001,102.073/<0.001).Among the positive SPP1 expression in NSCLC patients,the percentage of patients with tumor diameter≥5 cm,TNM staging in 35 years,lymph node metastasis and history of smoking was higher(χ^(2)/P=5.408/0.020,10.636/<0.001,6.838/<0.001,10.352/<0.001).At the same time,the proportion of these patients in SLC7A11 positive expression was also higher(χ^(2)=6.965,7.638,10.597,16.657,P<0.001),while in MTSS1 positive expression,The proportion of patients with TNM stageⅠtoⅡ,no lymph node metastasis and no smoking history was higher(χ^(2)=9.681,10.611,7.187,P<0.001).Compared with the survival group,the proportion of smoking history and occupational exposure,TNM stageⅢand lymph node metastasis in the death group was significantly higher(χ^(2)/P=7.501/<0.001,4.205/0.040,12.861/<0.001,10.907/<0.001).The survival rate of SPP1 and SLC7A11 positive patients in the death group was lower than that of the survival group,while the survival rate of MTSS1 positive patients in the survival group was higher than that of the death group(χ^(2)/P=11.273/<0.001,11.490/<0.001,7.603/0.006).Multivariate Cox regression results showed that TNM stageⅢ,lymph node metastasis,positive SPP1,positive SLC7A11 and smoking history were all risk factors for poor prognosis of NSCLC.Positive MTSS1 was a protective factor for poor prognosis of NSCLC[HR(95%CI)=2.369(1.623-3.458),1.843(1.308-2.597),2.014(1.396-2.906),1.986(1.420-2.777),2.259(1.370-3.724),0.856(0.754-0.972)].The three-year overall survival rate of NSCLC patients with SPP1 and SLC7A11 positive expression was lower than that of SPP1 and SLC7A11 negative expression(Log Rank χ^(2)/P=9.988/0.002,10.608/0.001),respectively.The three-year overall survival rate of patients with positive expression of MTSS1 was higher than that with negative expression of MTSS1(Log Rank χ^(2)/P=7.884/0.005).Conclusion SPP1 and SLC7A11 have a high positive expression rate in NSCLC tissues,while MTSS1 has a low positive expression rate in NSCLC tissues.The expression of these three proteins is closely related to the pathological features and prognosis of NSCLC.
作者
罗红兰
刘凯
童凯
潘丽钧
周冬
陈畇尚
Luo Honglan;Liu Kai;Tong Kai;Pan Lijun;Zhou Dong;Chen Yunshang(Department of Oncology,Huanggang Central Hospital,Hubei,Huanggang 438000,China;不详)
出处
《疑难病杂志》
2025年第9期1056-1062,共7页
Chinese Journal of Difficult and Complicated Cases
基金
国家自然科学基金资助项目(82303828)。
关键词
非小细胞肺癌
磷蛋白1
转移抑制因子1
溶质载体家族7成员11
预后
Non-small cell lung cancer
Phosphoprotein 1
Metastasis suppressor 1
Solute carrier family 7 member 11
Prognosis
