摘要
工业规模的光自养微藻生物质中油脂含量低,无法满足制生物柴油要求,培养过程缺少指导,且现有理论模型在工业规模下缺乏应用验证。为了对大型工业光反应器生产过程进行预测和调整,分析探讨了高含油量微拟球藻培养的技术难点和改进措施,基于一种光吸收系数(f2)估测方法和最大生物质产量的理论模型,提出了一套低成本高含油量微藻培养方案,并在工程中进行实践检验。实践表明:在以跑道池26为光反应器,藻液高度15cm,氮供应量为改良f/2培养基的1/9条件下,成功生产出油脂含量高于30%的微拟球藻藻粉。综上,所建立的微藻光合生长预测模型可行,通过调整跑道池藻液高度和培养基氮元素含量,可生产出高含油量微拟球藻藻粉。
Industrial-scale photoautotrophic microalgae biomass has low oil content,which cannot meet the requirements for biodiesel production.The cultivation process lacks guidance,and existing theoretical models have not been verified for industrial-scale applications.To predict and adjust the production process of large-scale industrial photobioreactors,the technical challenges and improvement measures for producing high oil content Nannochloropsis oceanica biomass were analyzed and discussed.Based on a light absorption coefficient(f2)estimation method and theoretical model of maximum biomass yield,a low-cost cultivation scheme to produce high oil content N.oceanica biomass was proposed,which was tested in a factory.Practice showed that under the conditions of runway pool 26 as a photoreactor,algal solution height of 15 cm,and nitrogen supply of 1/9 modified f/2 medium,the algal powder of N.oceanica with oil content higher than 30%was successfully produced.In conclusion,the established model for predicting the photosynthetic growth of N.oceanica is feasible,and by adjusting the height of the algal solution in the runway pool and the nitrogen content of the culture medium,the algal powder of N.oceanica with high oil content can be produced.
作者
黄聪
何荣
魏帅
陈湛平
李俊
杨黄鹏
王珍懿
辛凯
程军
HUANG Cong;HE Rong;WEI Shuai;CHEN Zhanping;LI Jun;YANG Huangpeng;WANG Zhenyi;XIN Kai;CHENG Jun(Guangdong Yuedian Zhanjiang Biomass Power Co.,Ltd.,Zhanjiang 524300,Guangdong,China;Guangdong Yuedian Environmental Protection Co.,Ltd.,Zhanjiang 510630,Guangdong,China;State Key Laboratory of Clean Energy Utilization,Zhejiang University,Hangzhou 310027,China;Key Laboratory of Low-grade Energy Utilization Technologies and Systems of Ministry of Education,Chongqing University,Chongqing 400044,China)
出处
《中国油脂》
北大核心
2025年第9期101-106,123,共7页
China Oils and Fats
基金
广东省重点领域研发计划(2022B0111130003)
浙江省领雁研发攻关计划(2024C03110)。
关键词
理论模型
高含油量
生物质产量
微拟球藻
工业生产
theoretical model
high oil content
biomass yield
N.oceanica
industrial production
