摘要
为改善红曲米中红曲黄色素(Monascus yellow pigment,MYP)水溶性低和靶向性差的问题,提高其生物利用度,本实验从红曲米粉中提取MYP,在此基础上进一步制备了MYP-玉米醇溶蛋白-半乳糖基化壳聚糖(galactosylated chitosan,GC)纳米颗粒(MYP-GC-NP),并评估了其对油酸/棕榈酸诱导HepG2细胞脂质积累的改善作用。结果表明,MYP-GC-NP呈球形且均匀分布,其粒径为(167.00±0.60)nm,多分散性指数为0.25±0.01,ζ-电位为70.88 mV。此外,与未被半乳糖基化壳聚糖修饰的纳米颗粒相比,MYP-GC-NP更容易被肝细胞摄取,并能够显著减少细胞内脂质沉积。本研究可为改善MYP生物利用度提供新的思路,并为MYP的纳米颗粒在改善脂质沉积方面的应用提供理论依据。
To address the poor water solubility and low targeting ability of Monascus yellow pigment(MYP)from Monascus rice and thereby enhance its bioavailability,this study prepared MYP-zein-galactosylated chitosan nanoparticles(MYP-GC-NP)and evaluated its efficacy in ameliorating oleic acid/palmitic acid(OA/PA)-induced lipid accumulation in HepG2 cells.The results showed that MYP-GC-NP exhibited a spherical shape and uniform size distribution with an average particle size of(167.00±0.60)nm,a polydispersity index(PDI)of 0.25±0.01,and aζ-potential of 70.88 mV.Additionally,compared with nanoparticles without modification by GC,MYP-GC-NP were more readily taken up by hepatocytes and significantly reduced intracellular lipid accumulation.This study provides a novel strategy to improve the bioavailability of MYP and offers a theoretical basis for the application of MYP-loaded nanoparticles in alleviating lipid accumulation.
作者
林杨
孙思邈
信文娟
毛延卿
陈绕
邵平
LIN Yang;SUN Simiao;XIN Wenjuan;MAO Yanqing;CHEN Rao;SHAO Ping(College of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China;Zhejiang Jolly Pharmaceutical Co.,Ltd.,Huzhou 313200,China;Zhejiang Johncan Mushroom Biotechnology Co.,Ltd.,Huzhou 313200,China;Hangzhou Zixiang Sugar Co.,Ltd.,Hangzhou 311200,China;Moganshan Institute,Zhejiang University of Technology,Huzhou 313200,China)
出处
《食品科学》
北大核心
2025年第20期170-179,共10页
Food Science
基金
国家自然科学基金青年科学基金项目(32102006)
浙江省科技计划项目(2023C04007)。
关键词
红曲黄色素
纳米颗粒
半乳糖基化壳聚糖
脂质沉积
Monascus yellow pigment
nanoparticles
galactosylated chitosan
lipid deposition
