摘要
本研究对湖南省儿童医院检验中心2021年1月至2022年12月收集的23株儿童黏液型流感嗜血杆菌进行全基因组测序、血清型分型、多位点序列分型(MLST)分析及群体遗传结构分析、核心基因组单核苷酸多态性位点(cgSNP)分析。采用全自动微生物鉴定药敏分析系统AF-600进行药敏实验,并基于全基因组序列进行毒力基因及耐药基因分析。基于全基因测序结果表明,23株黏液型Hi均为b型流感嗜血杆菌(Hib)。MLST分型共检测到6种ST型,其中ST386共10株(43.5%),ST18共5株(21.7%),新发现ST2887共2株(8.7%);贝叶斯推断的群体结构分析(BAPS)显示有3个不同的种群。毒力基因结果显示,编码荚膜相关蛋白基因、与细菌免疫调节和炎症信号通路相关等基因携带率为100%,而与细菌黏附相关的ompP5基因、与细菌蛋白代谢相关的hgpC和hxuA基因,均存在于新发现的ST2887中;23株黏液型Hib的体外药物敏感性试验结果表明,对氨苄西林、头孢呋辛和甲氧苄啶-磺胺甲噁唑的耐药率分别为13.0%(3株)、13.0%(3株)和87.0%(20株);对其他β-内酰胺类药物均敏感。综上,本研究发现23株儿童感染黏液型流感嗜血杆菌均为Hib,ST分型主要为ST386、ST18。
Whole-genome sequencing,serotype typing,MLST typing,population genetic structure analysis,and core-genome single nucleotide polymorphism(cgSNP)analysis were performed on 23 strains of mucoid Haemophilus influenzae collected from the Laboratory Center of Hunan Children′s Hospital from January 2021 to December 2022.Drug susceptibility tests were conducted using the automated microbial identification and susceptibility analysis system AF-600,and virulence genes and drug resistance genes were analyzed based on the whole-genome sequences.Based on the serotype typing of whole-genome sequencing,all 23 strains were Haemophilus influenzae type b(Hib).MLST analysis revealed that the 23 strains belonged to six ST types,among which 10 strains(43.5%)were ST386,5 strains(21.7%)were ST18,and 2 strains(8.7%)were the newly discovered ST2887.Bayesian population structure analysis(BAPS)showed three distinct populations.The results of the virulence gene analysis showed that the carriage rates of genes encoding capsule-related proteins and those associated with bacterial immune regulation and inflammatory signaling pathways were all 100%.However,the ompP5 gene related to bacterial adhesion,and the hgpC and hxuA genes related to bacterial protein metabolism were all present in the newly discovered ST2887.The in vitro drug susceptibility test results of 23 strains of mucoid Hib showed that the resistance rates to ampicillin,cefuroxime,and Trimethoprim-sulfamethoxazole were 13.0%(3 strains),13.0%(3 strains),and 87.0%(20 strains),respectively.They were sensitive to other beta-lactam drugs.All the 23 strains of mucoid Haemophilus influenzae infected children were Hib,and the main ST types were ST386 and ST18.It is very important to advocate Hib vaccination.
作者
刘健龙
邓美
宋春荣
付敏
刘雅
黄彩芝
匡敏
王小妹
Liu Jianlong;Deng Mei;Song Chunrong;Fu Min;Liu Ya;Huang Caizhi;Kuang Min;Wang Xiaomei(Laboratory Center,Hunan Children′s Hospital,Changsha 410007,China;Department of Laboratory Medicine,the First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)
出处
《中华预防医学杂志》
北大核心
2025年第8期1287-1293,共7页
Chinese Journal of Preventive Medicine
基金
湖南省自然科学基金项目(2023JJ60011)。
关键词
儿童
B型流感嗜血杆菌
基因组测序分型
毒力基因
Children
Haemophilus influenzae type b
Genome sequencing and typing
Virulence gene
