摘要
目的研究柴胡桂枝干姜汤治疗慢性非萎缩性胃炎的物质基础及疗效机制。方法采用UHPLC-QTOF/MS技术,获取柴胡桂枝干姜汤体外及入血化学成分的一级、二级离子碎片,结合对照品及文献上的信息进行比对,对柴胡桂枝干姜汤的化学成分进行全面表征。在入血成分表征的基础上,利用网络药理学方法,对柴胡桂枝干姜汤治疗慢性非萎缩性胃炎的机制进行探究,并通过免疫组化结果进行验证。结果柴胡桂枝干姜汤的入血成分表征出24种,包括黄酮苷类11种,脂肪酸类3种,有机酸类3种,姜辣素类2种,黄酮类2种,三萜类、脂肪醛类和氨基酸类各1种。其主要作用于TNF-α、IL-6、STAT3以及PTGS2等靶点,通过影响IL-17信号通路,AGE-RAGE信号通路等发挥治疗作用。结论首次利用UHPLC-Q-TOF/MS对柴胡桂枝干姜汤治疗慢性非萎缩性胃炎的物质基础及疗效机制进行探索,为柴胡桂枝干姜汤的进一步研究提供科学参考。
Objective To investigate the effective components and therapeutic mechanism of Chaihu-Guizhi-Ganjiang decoction in treating chronic non-atrophic gastritis.Methods The primary and secondary ion fragments of chemical components of Chaihu-Guizhi-Ganjiang decoction were obtained by UHPLC-Q-TOF/MS.Comparing with reference standards and literature information,a comprehensive characterization of the chemical constituents of Chaihu-Guizhi-Ganjiang decoction was conducted.Then,the network pharmacology approach was applied to explore the therapeutic mechanism of Chaihu-Guizhi-Ganjiang decoction in treatment of chronic non-atrophic gastritis based on the components in plasma and verified by immunohistochemical results.Results A total of 24 absorbed components of Chaihu-Guizhi-Ganjiang decoction were characterized,including 11 flavonoid glycosides,3 fatty acids,3organic acids,2 gingerols,2 flavonoids and,1 each of fatty aldehydes,triterpenoids and amino acids,which mainly acted on TNF-α,IL-6,STAT3,and PTGS2.It exerted therapeutic effects by modulating signaling pathways,including the IL-17 signaling pathway and the AGE-RAGE signaling pathway,etc.Conclusion This study provided the first exploration of the effective components and therapeutic mechanism of Chaihu-Guizhi-Ganjiang decoction in treatment of chronic non-atrophic gastritis by UHPLC-Q-TOF/MS,which could offer scientific references for its further research.
作者
闻韬
焦广洋
赵梦沛
岳小强
张凤
陈万生
WEN Tao;JIAO Guangyang;ZHAO Mengpei;YUE Xiaoqiang;ZHANG Feng;CHEN Wansheng(Department of Pharmacy,Medical Security Center,The Second Affiliated Hospital of Naval Medical University,Shanghai 200003,China;Department of Traditional Chinese Medicine,The Second Affiliated Hospital of Naval Medical University,Shanghai 200003,China;Institute of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《药学实践与服务》
2025年第9期455-462,共8页
Journal of Pharmaceutical Practice and Service
