摘要
目的本研究旨在探索丁香酚联合中医五行音乐疗愈对2,4-二硝基氟苯(2,4-dinitrofluorobenzene,DNFB)诱导的特应性皮炎(AD)小鼠模型的皮肤炎症、行为学特征、神经递质与激素水平及肠道菌群的改善。方法通过连续21 d使用DNFB诱导建立小鼠AD模型,期间记录小鼠背部皮损变化及瘙痒次数。第19、20天进行旷场实验、高架0迷宫实验、悬尾实验和强迫游泳实验。第21天处死并解剖小鼠,计算脏器指数。同时,采用ELISA法测定血清中IgE、IL-4和IL-10水平,取背部皮肤切片进行苏木素-伊红(HE)、甲苯胺蓝染色观察皮肤病理变化,测量小鼠脑中皮质酮、多巴胺和5-羟色胺水平。此外,采用16S rRNA基因测序分析小鼠肠道菌群组成。结果与DNFB诱导的AD小鼠相比,丁香酚联合中医五行音乐疗愈(简称联合)组显著降低了皮损评分,减少了皮肤炎性细胞浸润。在行为学测试中,联合组小鼠表现出更活跃的运动状态,增加了对陌生环境的探索欲望,同时增加了在压力情境下的挣扎时间。神经递质与激素检测结果显示,该联合疗法提高了小鼠脑内5-羟色胺、多巴胺和皮质酮的水平。此外,16S rRNA基因测序结果显示,DNFB诱导的AD小鼠肠道菌群多样性发生变化,而联合组重塑了肠道微生物组成,某些肠道微生物如利基乳酸菌属(Ligilactobacillus)和毛螺菌属(Lachnospiraceae)的相对丰度增加,而副拟杆菌属(Parabacteroides)和拟杆菌属(Bacteroides)的相对丰度减少。Spearman相关性分析发现,改变的肠道微生物群、AD相关的皮肤表现和共病产生的焦虑抑郁样行为之间存在显著的相关性。结论丁香酚联合中医五行音乐疗愈通过调节AD小鼠肠道菌群、改善行为学表现,对共病焦虑和抑郁样行为产生了积极影响,为AD及其焦虑抑郁样行为的改善提供了新的思路和方法。
Objective To explore the effects of eugenol combined with traditional Chinese medicine Wuxing music(EUG+MUSIC)on skin inflammation,behavioral characteristics,hormone and neurotransmitter levels,and the intestinal microbiota in a mouse model of 2,4-dinitrofluorobenzene(DNFB)-induced atopic dermatitis(AD).MethodsA mouse model of AD was established by induction with DNFB for 21 consecutive days;throughout the study,changes in dorsal skin lesions and scratching behavior were recorded in mice and changes in dorsal skin lesions and pruritus were recorded.Mice underwent open-field,elevated 0 maze,tail-suspension,and forced-swimming tests on days 19 and 20.The mice were then sacrificed on day 21 and the organ index was calculated.Serum levels of immunoglobulin E,interleukin(IL)-4,and IL-10 were determined by enzyme-linked immunosorbent assay.Pathological changes in back-skin sections were detected by hematoxylin-eosin(HE)and toluidine blue staining,and brain levels of corticosterone,dopamine,and serotonin were measured.The composition of the intestinal microbiota was analyzed by 16S rRNA gene sequencing.ResultsThe skin lesion score and skin inflammatory cell infiltration were significantly reduced in the EUG+MUSIC group.Regarding the behavioral tests,mice in the EUG+MUSIC group showed more active locomotion,an increased desire to explore unfamiliar environments,and increased struggling time in stressful situations.Brain levels of serotonin,dopamine,and corticosterone were also increased in the EUG+MUSIC group.16S rRNA gene sequencing showed that DNFB affected the diversity of the intestinal microbiota in AD mice,while EUG+MUSIC treatment remodeled the gut microbial composition,including increasing the relative abundance of gut microbes such as Ligilactobacillus and Lachnospiraceae and decreasing Parabacteroides and Bacteroides spp.Spearman’s analysis showed significant correlations among altered gut microbiota,AD-associated skin manifestations,and anxiety-and depressive-like behaviors arising from comorbidities.ConclusionsEugenol combined with traditional Chinese medicine five-elements music therapy may have a positive effect on comorbid anxiety-and depression-like behaviors by regulating the intestinal microbiota and improving the behavioral performance in mice with AD,thus providing new ideas and method for improving AD and its associated anxiety-and depressive behaviors.
作者
刘佩荣
王丁文
陈悦
张颖
曹晓琴
LIU Peirong;WANG Dingwen;CHEN Yue;ZHANG Ying;CAO Xiaoqin(Department of Music,School of Arts,Hubei Polytechnic University,Huangshi 435003,China;College of Pharmacy,School of Medicine,Jianghan University,Wuhan 430100,China)
出处
《中国实验动物学报》
北大核心
2025年第8期1116-1132,共17页
Acta Laboratorium Animalis Scientia Sinica
基金
江汉大学2025年省级大学生创新训练项目(S202511072087)。
关键词
特应性皮炎
丁香酚
五行音乐疗愈
焦虑抑郁样行为
肠道菌群
atopic dermatitis
eugenol
five-elements music therapy
anxiety-and depressive-like behavior
gut microbiota
