摘要
Single-molecule localization microscopy(SMLM)has pushed resolution to sub-40 nm.Combined with structured illumination,lateral resolution can be doubled or the axial resolution can be improved fourfold.However,current techniques are challenging in balancing the lateral and axial resolutions.Here,we report a new modulated illumination single-molecule localization modality,isoFLUX.Utilizing two objective lenses to form interference patterns along the x-z and y-z directions,the lateral and axial resolutions can be improved simultaneously.Compared to SMLM,isoFLUX maintains a twofold average enhancement in both lateral and axial resolutions under an astigmatic point spread function(PSF),1.5-fold in the lateral resolution and 2.5-fold in the axial resolution under the saddle-point PSF.
基金
supported by the National Natural Science Foundation of China(No.62305320)。
