摘要
卡松作为日化行业一种广泛使用的防腐剂,其耐药性腐败菌株的存在可能导致化妆品等产品腐败变质,对消费者健康构成威胁。为了探究卡松耐药菌的耐药机制,特别是伯克霍尔德氏菌属的耐药性,文章分离并驯化了洋葱伯克霍尔德氏菌JKZ-12菌株,对其进行转录组测序分析,并运用qRT-PCR技术进行检验。结果表明,在卡松胁迫下,该菌株的鞭毛和趋化性相关基因表达量普遍增加,揭示菌株可能通过增强趋化性运动来逃避高浓度卡松环境;铁载体等物质跨膜运输相关基因表达量的改变,可能导致胞内卡松浓度下降;多种酶活性和物质代谢的变化,可能与耐药性直接或间接相关。文章揭示了洋葱伯克霍尔德卡松耐药性的发生与鞭毛和趋化性运动、铁载体转运系统的相关性,为理解卡松耐药机制提供了新方向,并为未来开发新的抗菌策略奠定了基础。
Isothiazolinone(Kathon),a widely used preservative in daily chemical industry,can lead to product spoilage for cosmetics and health hazards for consumers when its drug-resistant putrefying bacterial strains are present.The paper aimed to investigate the resistance mechanisms of isothiazolinone-resistant bacteria,particularly focusing on the resistance of Burkholderia.Isolation and domestication of the Burkholderia cepacia strain JKZ-12 were performed,followed by transcriptome sequencing analysis,and the results were verified using qRT-PCR.The results indicated that under isothiazolinone stress,the expression levels of the flagella and chemotaxis-related genes of the strain are generally increased,suggesting that the bacteria may enhance their chemotactic motility to evade high concentrations of isothiazolinone.The expression of genes related to transmembrane transport,including siderophore,was altered,which may lead to a decrease in intracellular isothiazolinone concentration.Various enzyme activities and metabolic processes were altered,which may be directly or indirectly related to resistance.The papar revealed that isothiazolinone resistance of B.cepacia may be associated with flagella,chemotactic motility and siderophore transport systems,which provides new insights into the mechanisms of isothiazolinone resistance and lays the foundation for the development of novel antibacterial strategies in the future.
作者
曹畅达
许炎坤
温灼斌
刘永龙
周刚
李良秋
CAO Changda;XU Yankun;WEN Zhuobing;LIU Yonglong;ZHOU Gang;LI Liangqiu(Guangdong Dimei New Materials Technology Co.Ltd.,Zhaoqing 526200,China;Guangdong Dimei Biological Technology Co.Ltd.,Guangzhou 510030,China;Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510030,China)
出处
《工业微生物》
2025年第4期193-201,共9页
Industrial Microbiology
关键词
卡松
耐药性
转录组测序
洋葱伯克霍尔德氏菌
Kathon
drug resistance
transcriptome sequencing
Burkholderia cepacian
