摘要
为构建凤梨释迦组培快繁体系,本试验以该植物的幼嫩带节茎段为外植体,分析不同激素配比的培养基对其愈伤组织的诱导率、分化率,不定芽的增殖倍数以及生根率的影响。结果表明,凤梨释迦组培最佳的愈伤组织诱导培养基为MS+6-BA 0.5 mg/L+2,4-D 2 mg/L+蔗糖30 g/L+琼脂6 g/L,诱导率达62.50%;最佳分化培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+蔗糖30 g/L+琼脂6 g/L,分化率达65.00%;最佳增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+蔗糖30 g/L+琼脂6 g/L,增殖系数为4.2;最佳生根培养基为1/2 MS+NAA 0.2 mg/L+蔗糖20 g/L+琼脂6 g/L,生根率达83.75%;组培苗移栽成活率在90%以上。综上,本研究建立了凤梨释迦组培繁殖关键技术体系,为其种苗工厂化、标准化生产提供参考。
To establish a rapid propagation system for the tissue culture of Annona×atemoya,in this experiment,the young and segmented stem segments of the plant were used as explants to determine the induction rate and differentiation rate of callus,the proliferation multiple of adventitious buds and the rooting rate of media with different hormone ratios.The results showed that the optimal callus induction medium for tissue culture of Annona×atemoya was MS+6-BA 0.5 mg/L+2,4-D 2 mg/L+sucrose 30 g/L+agar 6 g/L,with an induction rate of 62.50%.The optimal differentiation medium was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+sucrose 30 g/L+agar 6 g/L,with a differentiation rate of 65.00%.The optimal proliferation medium is MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+sucrose 30 g/L+agar 6 g/L,with a proliferation coefficient of 4.2.The optimal rooting medium is 1/2 MS+NAA 0.2 mg/L+sucrose 20 g/L+agar 6 g/L,with a rooting rate of 83.75%.The survival rate of tissue culture seedlings after transplantation is over 90%.In conclusion,this study established a key technical system for tissue culture propagation of Annona×atemoya,providing a reference for the factory and standardized production of sugar apple seedlings.
作者
武爱龙
杨丽冬
黄岳平
庞凯云
李颖华
WU Ailong;YANG Lidong;HUANG Yueping;PANG Kaiyun;LI Yinghua(Zhanjiang Preschool Teachers College,Zhanjiang 524000,China)
出处
《安徽农学通报》
2025年第17期50-53,共4页
Anhui Agricultural Science Bulletin
基金
湛江幼儿师范专科学校教学质量与教学改革工程项目“产教融合视域下高职高专院校创新创业教育实践模式的构建”(ZLGC202410)
广东省普通高校特色创新项目“乡村振兴背景下构建湛江地区释迦果种苗繁育体系”(2023KTSCX379)。
关键词
凤梨释迦
组织培养
快繁体系
愈伤组织
Annona×atemoya
tissue culture
rapid propagation system
callus induction
