摘要
目的:探讨CD19/CD73在慢性淋巴细胞白血病(CLL)中的表达及与临床特征的相关性。方法:回顾性分析2022年1月至2023年11月我院收治的60例CLL患者以及40例同期健康志愿者(对照组)的临床资料,流式细胞术检测CLL患者外周血CD19、CD73的水平,并用Kaplan-Meier法对患者进行生存分析。结果:CLL组的血红蛋白、CD19/CD73均显著低于对照组,而CD19、CD73、β_(2)-MG显著高于对照组(均P<0.001)。经ROC曲线分析,CD19/CD73对CLL诊断的AUC值为0.980(95%CI:0.949-1.000,P<0.05),特异度为92.50%,敏感度为98.30%。脾肿大的CLL患者的CD19/CD73明显小于非脾肿大的患者(P<0.01)。CLL患者CD19/CD73与血红蛋白无显著相关性(r=0.056,P>0.05),与β_(2)-MG存在正相关性(r=0.837,95%CI:0.7402-0.8996,P<0.01)。根据CD19/CD73的中位数12.84将患者分为高、低表达两组,Kaplan-Meier生存曲线分析结果显示,低表达组18个月的总生存率和无进展生存率分别为87.08%、93.25%,高表达组分别为96.41%、99.90%,两组比较差异有统计学意义(均P<0.05)。结论:CD19/CD73在CLL患者机体中呈低水平,可作为CLL临床诊断的辅助指标,CD19/CD73与患者脾肿大密切相关,CD19/CD73低表达预示预后不良。
Objective:To investigate the expression of CD19/CD73 in chronic lymphocytic leukemia(CLL)and its correlation with clinical features.Methods:The clinical data of 60 CLL patients and 40 healthy volunteers(control group)from January 2022 to November 2023 were retrospectively analyzed.The levels of CD19 and CD73 in peripheral blood of CLL patients were measured by flow cytometry.Kaplan-Meier method was used for survival analysis.Results:The hemoglobin(Hb)and CD19/CD73 levels in CLL group were significantly lower than those in control group,while CD19,CD73 andβ_(2)-MG were significantly higher(all P<0.001).According to ROC curve analysis,the AUC value of CD19/CD73 for CLL diagnosis was 0.980(95%CI:0.949-1.000,P<0.05),the specificity was 92.50%,and the sensitivity was 98.30%.The CD19/CD73 level of CLL patients with splenomegaly was significantly lower than those without splenomegaly(P<0.01).There was no significant correlation between CD19/CD73 and Hb in CLL patients(r=0.056,P>0.05).CD19/CD73 was positively correlated withβ_(2)-MG(r=0.837,95%CI:0.7402-0.8996,P<0.01).According to the median value(12.84)of CD19/CD73,the patients were divided into high and low expression groups.Kaplan-Meier survival analysis showed that the overall survival rate and progression-free survival rate at 18 th month in the low expression group were 87.08%and 93.25%,while those in the high expression group were 96.41%and 99.90%,respectively(both P<0.05).Conclusion:The level of CD19/CD73 is low in CLL patients,which can be used as an auxiliary index for clinical diagnosis of CLL.CD19/CD73 is closely related to splenomegaly in CLL patients.Low expression of CD19/CD73 predicts poor prognosis.
作者
汪彦屿
刘兰
赵玉洁
石耿辉
闵迅
WANG Yan-Yu;LIU Lan;ZHAO Yu-Jie;SHI Geng-Hui;MIN Xun(Department of Laboratory,The Affiliated Hospital of Zunyi Medical University,Zunyi 563003,Guizhou Province,China;Department of Hematology,The Affiliated Hospital of Zunyi Medical University,Zunyi 563003,Guizhou Province,China)
出处
《中国实验血液学杂志》
北大核心
2025年第5期1274-1278,共5页
Journal of Experimental Hematology
