摘要
[目的]探讨中草药提取物对奶牛隐性乳腺炎的防控效果。[方法]选取24头隐性乳腺炎奶牛,随机分为4组。对照组给予正常饲料,试验组分别饲喂20 g/d、40 g/d和80 g/d的中草药提取物。记录各组奶牛产奶量、奶质量,以及牛髓过氧化物酶(MPO)、乳酸脱氢酶(LDH)、谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、白介素-6(IL-6)、白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的含量变化。[结果]饲喂40 g/d和80 g/d中草药提取物的奶牛,其产奶量、乳糖率、乳蛋白率显著提高,体细胞数显著减少,同时MPO和LDH的含量下降。此外,GSH、SOD和CAT活性增强,MDA含量降低,炎症因子IL-6、IL-1β和TNF-α的表达显著抑制。[结论]中草药提取物能够有效提高隐性乳腺炎奶牛的产奶量和奶质量,调节乳中活性酶含量,增强抗氧化能力,抑制炎症因子的表达,有良好的防控效果。
[Objective]This study aimed to investigate the preventive and therapeutic effects of Chinese herbal medicine extract(CHME)on subclinical mastitis in dairy cows.[Method]Twenty-four dairy cows with subclinical mastitis were randomly divided into four groups.The control group received a standard diet,while the three treatment groups were supplemented with CHME at 20 g/d,40 g/d,and 80 g/d,respectively.Milk yield,milk composition(including lactose percentage,protein percentage,and somatic cell count),and the levels of key biomarkers were recorded.These biomarkers included myeloperoxidase(MPO),lactate dehydrogenase(LDH),glutathione peroxidase(GPX),superoxide dismutase(SOD),catalase(CAT),malondialdehyde(MDA),interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α).[Result]Cows supplemented with 40 g/d and 80 g/d of CHME exhibited a significant increase in milk yield,lactose percentage,and protein percentage,along with a significant reduction in somatic cell count.Concurrently,the levels of MPO and LDH decreased.Furthermore,the activities of the antioxidant enzymes GPX,SOD,and CAT were enhanced,while MDA content decreased.The expression of the pro-inflammatory cytokines IL-6,IL-1β,and TNF-αwas significantly suppressed.[Conclusion]Chinese herbal medicine extract effectively improved milk yield and quality in cows with subclinical mastitis.It regulated the levels of active enzymes in milk,enhanced antioxidant capacity,and inhibited the expression of inflammatory cytokines,demonstrating good preventive and therapeutic effects.
作者
黄晶
HUANG Jing(Cao County Livestock Service Center,Heze Shandong 274400)
出处
《中国乳业》
2025年第8期47-53,共7页
China Dairy
关键词
隐性乳腺炎
奶牛
产奶量
体细胞数
subclinical mastitis
dairy cows
milk yield
somatic cell count
