摘要
干旱胁迫严重制约菊花脑(Chrysanthemum nankingense)的生长发育,解析其耐旱分子机制对菊花品种改良具有较重要的意义。以10叶期菊花脑幼苗为材料,通过40%PEG-6000模拟干旱处理,结合转录组测序技术探究其响应干旱的分子调控网络。结果显示,干旱胁迫诱导了848个差异表达基因(differentially expressed genes,DEGs),其中,484个显著上调,364个下调。基因本体(Gene Ontology,GO)、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析表明,DEGs主要参与脱落酸信号响应、脱水应激、活性氧清除及次生代谢物合成等生物学过程。木质素生物合成关键基因(PAL、F5H、CST、CCoAOMT、CAD)在干旱组中表达量显著提升,顶端和基部茎秆的木质素含量较对照组分别增加1.98倍和1.38倍,表明木质化增强可能是菊花脑适应水分缺失的重要策略。研究首次系统揭示了菊花脑在干旱响应中多通路协同调控的分子框架,能为耐旱基因挖掘及分子育种提供理论依据。
Drought stress severely constrains the growth of Chrysanthemum nankingense(C.nankingense),and elucidating its drought tolerance mechanisms holds significant implications for chrysanthemum breeding.Using C.nankingense 10-leaf-stage seedlings subjected to 40%PEG-6000-simulated drought,this study investigates molecular regulatory networks via transcriptome sequencing.Results identify 848 differentially expressed genes(DEGs),with 484 significantly up-regulated and 364 down-regulated.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses demonstrate DEG involvement in abscisic acid signaling,dehydration response,reactive oxygen species scavenging,and secondary metabolite synthesis.Key lignin biosynthesis genes(PAL,F5H,CST,CCoAOMT,CAD)exhibit markedly elevated expression under drought,correlating with 1.98-fold and 1.38-fold increases in lignin content in apical and basal stems versus controls.These findings suggest enhanced lignification constitutes a critical adaptive strategy to water deficit in C.nankingense.This research systematically reveals a multi-pathway coordinated molecular framework for drought response in C.nankingense,providing a theoretical basis for drought-tolerance gene mining and molecular breeding.
作者
王涛
王婉艺
邵倩
毕淑觃
韩乾坤
范翔龙
任辉
张小龙
WANG Tao;WANG Wanyi;SHAO Qian;BI Shuyan;HAN Qiankun;FAN Xianglong;REN Hui;ZHANG Xiaolong(Department of Biology and Food Engineering,Bozhou University,Bozhou 236800,China;Anhui Engineering Research Center for the Development and Application of Functional Blended Liquor,Bozhou 236800,China;Bozhou Key Laboratory of Biosynthesis of Effective Ingredients from Medicinal Plants,Bozhou 236800,China)
出处
《邵阳学院学报(自然科学版)》
2025年第4期63-73,共11页
Journal of Shaoyang University:Natural Sciences Edition
基金
安徽省高等学校科学研究项目(2024AH051312)
亳州市重点研发计划项目(bzzc2024035)
亳州学院2023年度科研启动项目(BYKQ202307)
亳州学院2024年度科研启动项目(BYKQ202421)
亳州学院横向项目(BYH202461)。
关键词
菊花脑
干旱胁迫
转录组
木质素
Chrysanthemum nankingense
drought stress
transcriptome
lignin
