摘要
试验旨在研究奶山羊精液冷冻保存稀释液中添加不同浓度的绿原酸(chlorogenic acid,CGA)对冷冻后精液品质的影响。在精液冷冻稀释液中分别添加0、0.2、0.4、0.8和1.2 mg/mL的CGA,利用细管法在液氮中冷冻精液,37℃解冻后检测精子的活力、运动学参数、顶体质膜完整性,抗氧化性能以及凋亡蛋白。结果表明,当添加0.4 mg/mL的CGA时,解冻后的精子活力、运动性能、顶体完整性和质膜完整性达到最高水平,明显高于对照组(P<0.05);在添加0.4 mg/mL的CGA时,精子的T-AOC、CAT和GSH-Px最高,并且明显高于对照组(P<0.05),MDA最低,明显低于对照组(P<0.05)。当CGA浓度为0.8 mg/mL时,SOD的最高值为,显著高于对照组(P<0.05);检测凋亡蛋白表达的水平,添加CGA的精子促凋亡蛋白a-Caspase-3表达降低,而Bcl-2抑制凋亡蛋白的表达上升,促凋亡蛋白BAX表达下降。总之,在奶山羊精液稀释液中添加0.4 mg/mL的CGA可以提高奶山羊冷冻精液的质量。
This study aimed to investigate the impact of adding different concentrations of chlorogenic acid(CGA)to the frozen diluent of goat semen on the quality of frozen semen.Different concentrations of CGA(0,0.2,0.4,0.8,and 1.2 mg/mL)were incorporated into the semen diluent,then cryopreserved in liquid nitrogen using a straw method.After thawing at 37℃,semen quality was assessed by measuring sperm viability,kinematic parameters,acrosome and plasma membrane integrity,antioxidant performance,and apoptosis proteins.The findings indicated that the addition of 0.4 mg/mL of CGA resulted in the highest levels of post-thaw sperm viability,motility,acrosome and plasma membrane integrity,which were significantly superior to the control group(P<0.05).Furthermore,at 0.4 mg/mL of CGA,the levels of total antioxidant capacity(T-AOC),catalase(CAT),and glutathione peroxidase(GSH-Px)in sperm were notably elevated,significantly surpassing those of the control group(P<0.05),while malondialdehyde(MDA)levels were notably reduced,significantly lower than the control group(P<0.05).Additionally,the highest superoxide dismutase(SOD)value was observed at a CGA concentration of 0.8 mg/mL,significantly higher than the control group(P<0.05).Analysis of apoptosis protein expression revealed that CGA supplementation decreased the expression of pro-apoptotic protein a-Caspase-3 in sperm,increased the expression of anti-apoptotic protein Bcl-2,and decreased the expression of pro-apoptotic protein BAX.In summary,the inclusion of 0.4 mg/mL of CGA in the diluent of dairy goat semen can enhance the quality of frozen semen from dairy goats.
作者
孙澳
郝璐
郑天明
徐鹏飞
韩晓燕
郭鑫
李宗儒
王满定
安小鹏
SUN Ao;HAO Lu;ZHENG Tianming;XU Pengfei;HAN Xiaoyan;GUO Xin;LI Zongru;WANG Manding;AN Xiaopeng(College of Animal Science and Technology,Northwest A&F University,Yangling Shaanxi 712100,China;Chunhua County Agriculture and Rural Bureau,Chunhua Shaanxi 711200,China;Heshui County Animal Disease Prevention and Control Center,Heshui Gansu 745400,China;Gucheng Town People's Government,Heshui Gansu 745400,China;Heshui County Agricultural Industry Development Investment Co.,Ltd,Heshui Gansu 745400,China;Xianyang Animal Disease Prevention and Control Center,Xianyang Shaanxi 712100,China)
出处
《家畜生态学报》
北大核心
2025年第8期66-71,共6页
Journal of Domestic Animal Ecology
基金
陕西省重点研发计划(2024NC-ZDCYL-03-01,2024NC-YBXM-082、2021ZDLNY02-01)
合水县奶羊高效养殖关键技术研发和推广(QY-HS-2023A-01)。
关键词
奶山羊
冷冻精液
绿原酸
抗氧化性能
凋亡途径
dairy goat
frozen semen
chlorogenic acid
antioxidant performance
apoptotic pathway
