摘要
为揭示磷对远志植物学性状、有效成分和根际土壤微生物群落的影响,该研究进行了磷肥施用实验。磷肥以P_(2)O_(5)计,设5个处理,分别为每亩(1亩≈667 m^(2)):0(CK)、17(P1)、34(P2)、51(P3)、68(P4)kg,采用随机区组设计,分别采集植株及其根际土壤,基于Illumina高通量测序分析根际土壤微生物群落,测定远志9个植物学性状,11个有效成分含量。结果表明,P1处理全株重、地上重、根重、根皮厚度达到最高值,较CK分别增加34.41%、38.80%、39.21%、3.17%,P2处理株高、株直径、根粗和木芯粗显著高于CK。磷肥对细叶远志皂苷、sibiricose A5、sibiricose A6、arillanin A、3,6′-二芥子酰基蔗糖与远志(口山)酮Ⅲ含量影响较大。细菌Arthrobacter、Bacillus、norank_f_Vicinamibacteraceae、norank_o_Vicinamibacterales、MND1和真菌Neocosmospora、Paraphoma等菌属与根粗、木芯粗、全株重、根重和地上重显著相关。Bacillus、Neocosmospora、Subulicystidium与3,6′-二芥子酰基蔗糖、sibiricose A5、sibiricose A6、glomeratose A、arillanin A和tenuifoliside C等寡糖酯正相关。Arthrobacter、Humicola、Aspergillus、Paraphoma与细叶远志皂苷正相关,norank_f_Vicinamibacteraceae、norank_o_Vicinamibacterales、Fusarium与远志(口山)酮Ⅲ正相关。可见,适宜施磷有利于远志生长与质量提升,并能增加土壤有益微生物丰度。
To investigate the effects of phosphorus fertilizer on the morphological traits,active ingredients and rhizosphere soil microbial community of Polygala tenuifolia.The phosphorus fertilizer was calculated in terms of P_(2)O_(5).Five treatments were set up:0(CK),17(P1),34(P2),51(P3),and 68(P4)kg per Mu(1 Mu≈667 m^(2)).A randomized block design was adopted.Samples of P.tenuifolia and its rhizosphere soil were collected under different superphosphate fertilizer treatments.Illumina high-throughput sequencing was used to analyze the rhizosphere soil microbial community,9 morphological traits were measured and the content of 11 active ingredients were determined.The results showed that the whole plant weight,shoot fresh weight,root weight,and root peel thickness were the highest under P1 treatment,increasing by 34.41%,38.80%,39.21%,and 3.17% respectively compared to CK.Under P2 treatment,the plant height,stem diameter,root thickness,and core thickness were significantly higher than CK.Phosphorus fertilizer had a significant impact on the content of tenuifolin,sibiricose A5,sibiricose A6,arillanin A,3,6′-disinapoyl sucrose,and polygalaxanthone Ⅲ.Correlation analysis results showed that the relative abundance of Arthrobacter,Bacillus,norank_f_Vicinamibacteraceae,norank_o_Vicinamibacterales,MND1 and other bacteria,as well as the relative abundance of Neocosmospora,Paraphoma and other fungi were positively correlated with root diameter,wood core diameter,the whole plant weight,root weight,shoot fresh weight of P.tenuifolia.Bacillus,Neocosmospora,Subulicystidium were significantly positively correlated with oligosaccharides such as 3,6′-disinapoyl sucrose,sibiricose A5、sibiricose A6、glomeratose A、arillanin A and tenuifoliside C.Arthrobacter,Humicola,Aspergillus,Paraphoma were positively correlated with tenuifolin and norank_f_Vicinamibacteraceae,norank_o_Vicinamibacterales,Fusarium were positively correlated with polygalaxanthone Ⅲ.Evidently,appropriate phosphorus application is conducive to the growth and quality improvement of P.tenuifolia,and can increase the abundance of beneficial microorganisms in the soil.
作者
郭欢
魏通
崔文华
石欢
毛福英
谷仙
赵云生
梁晓峰
CUO Huan;WEI Tong;CUI Wen-hua;SHI Huan;MAO Fu-ying;GU Xian;ZHAO Yun-sheng;LIANG Xiao-feng(College of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Hebei University of Chinese Medicine,Shijiazhuang 050091,China;Experimental Center,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;International Joint Research Center on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province,Shijiazhuang 050091,China;Mianyang Key Laboratory of Development and Utilization of Chinese Medicine Resources,Sichuan College of Traditional Chinese Medicine,Mianyang 621000,China)
出处
《中国中药杂志》
北大核心
2025年第14期3898-3908,共11页
China Journal of Chinese Materia Medica
基金
河北省自然科学基金中医药联合基金重点项目(H2023423019)
河北省重点研发计划“中药材现代种业科技创新团队”项目(21326312D)
河北省现代农业产业技术体系创新团队项目(HBCT2024110205,HBCT2024110201)。
关键词
远志
磷肥
植物学性状
有效成分
土壤微生物
Polygala tenuifolia
phosphorus fertilizer
morphological traits
active ingredients
soil microorganisms
