摘要
The liver is an immune organ,especially an immune tolerance organ.The critical shortage of donor organs and disease models for the treatment of end-stage liver failure underscores the urgent need for the generation of liver organoids from human induced pluripotent stem cells(iPSCs).Notably,significant advancements have been made in the study of liver organoids over the past decade.The construction of liver organoids has transitioned from single cell type to multicellular models,and from two-dimensional to three-dimensional cultures.Here we provide the progress surrounding the different liver organoids culture techniques from 3D printing to organ-onchip,as well as focuses on the present and future applications of liver organoids,and then to present challenges and perspectives ahead for further advancement.
基金
National Natural Science Foundation of China,Grant/Award Numbers:22211530067,22174121,T2250710180
2022Natural Science Foundation of Guangdong Provincial Basic and Applied Basic Research Fund
Guangdong Peral River Talent Program,Grant/Award Number:2021CX02Y066
Shenzhen Bay Open Laboratory Fund 2021
CUHKSZBoyalife Joint Laboratory Fund
China Postdoctoral ScienceFoundation,Grant/Award Number:2024M753133
University Development Fund,Grant/Award Number:UDF01002012
Shenzhen Science and Technology Program,Grant/Award Number:JCYJ20200109144201725
Guangdong Science and Technology Plan Project-Construction of High-level Biosafety Laboratories,Grant/Award Number:2021B1212030010。
