摘要
目的建立一种液相色谱-串联质谱法(LC-MS/MS)准确测定血浆Lyso-GL-3浓度。方法采用固相萃取技术处理血浆样本;在正离子模式,多反应监测(MRM)条件下,LC-MS/MS测定Lyso-GL-3浓度;验证方法的线性范围、检出限与定量限、正确度、精密度、基质效应、携带效应和血浆样本稳定性,并结合酶学和基因的检测结果来比对Lyso-GL-3阳性患者的准确度。结果Lyso-GL-3在1.25~400 nmol/L范围内线性良好,检出限为0.15 nmol/L、定量限为0.50 nmol/L,加标回收率为88.78%~108.96%,批内精密度、批间精密度、基质效应的变异系数(CV)均<15%,携带效应结果为0.55。血浆样本在冷藏条件下可稳定性保存30 d。临床符合度为100%。结论建立的血浆Lyso-GL-3浓度LC-MS/MS检测法,检测时间为2.5 min,简单快速,结果准确可靠。
ObjectiveTo establish a liquid chromatography tandem mass spectrometry(LC-MS/MS)method for accurate determination of plasma Lyso-GL-3 concentration.MethodSolid phase extraction technology was used to process plasma samples,and under positive ion mode and multiple reaction monitoring(MRM)conditions,LC-MS/MS was used to determine the concentration of Lyso-GL-3.The linear range,detection and quantification limits,accuracy,precision,matrix effect,carrier effect of the method,and plasma sample stability were validated.And the accuracy of Lyso-GL-3 positive patients was compared by combining enzymatic and genetic testing results.ResultLyso-GL-3 had good linearity in the range of 1.25-400 nmol/L.The detection limit and quantification limit were 0.15 nmol/L and 0.50 nmol/L,respectively.The spiked recovery rate was 88.78%-108.96%.The coefficient of variation(CV)for intra batch precision,inter batch precision,and matrix effect were all less than 15%,the result of carrier effect was 0.55%.Plasma samples could be stably stored for 30 days under refrigeration conditions.The clinical conformity of the patient was 100%.ConclusionThe established LC-MS/MS detection method for plasma Lyso-GL-3 concentration takes 2.5 minutes,which is simple,fast,accurate,and reliable.
作者
代冰
邱杰
邓雅妮
唐春燕
苏洁琼
杨琦
郭丹
余艳
Dai Bing;Qiu Jie;Deng Yani;Tang Chunyan;Su Jieqiong;Yang Qi;Guo Dan;Yu Yan(Changsha KingMed Center for Clinical Laboratory,Changsha 410005,China)
出处
《中华检验医学杂志》
北大核心
2025年第8期1041-1047,共7页
Chinese Journal of Laboratory Medicine
