摘要
为了探究隐蔽场所对鱼类的生长、个性和生理生化的影响,研究通过前期实验筛选出中华倒刺鲃(Spinibarbus sinensis)偏好的隐蔽场所(水草、树枝、阴影区域),设置了隐蔽场所组和无隐蔽场所组,进行为期60d的生长实验。生长实验结束后称量实验鱼体重、体长,并从每个养殖单元随机挑选5尾实验鱼取血浆测定皮质醇,取肝胰腺和脑测定抗氧化指标(T-AOC、SOD、CAT);其余实验鱼进行个性(探索性、活跃性、勇敢性和社会性)和焦虑样行为的测定。研究发现:(1)隐蔽场所组中华倒刺鲃生长速度显著高于无隐蔽场所组(最终体重增重35%,特定生长率增加18%,增重率提高45%);(2)隐蔽场所组中华倒刺鲃勇敢性显著高于无隐蔽场所组(P<0.05,且首次潜出时间缩短30.4%),探索性、活跃性和社会性无显著差异;(3)隐蔽场所对焦虑样行为无显著影响,但隐蔽场所组皮质醇水平显著低于无隐蔽场所组(P<0.001);(4)隐蔽场所组中华倒刺鲃肝胰腺SOD活力和T-AOC活力显著低于无隐蔽场所组(P<0.05),而其脑SOD活力则相反(P<0.05)。研究表明:虽然隐蔽场所对中华倒刺鲃的个性和焦虑样行为影响较小,但养殖环境隐蔽场所的设置可以促进中华倒刺鲃的生长速度、降低应激水平和抗氧化能力。
To investigate the impacts of shelters on growth,personality,and physiological responses in Spinibarbus sinensis,preferred shelters(weeds,branches,and shaded areas)identified through preliminary experiments were utilized.Fish were divided into a shelter group and a none shelter group for 60-day growth experiment.Subsequent measurements of fish body weight and length were conducted.We randomly selected 5 fishes to measure for plasma cortisol and antioxidant indices(total antioxidant capacity,T-AOC;superoxide dismutase,SOD;catalase,CAT)in the hepatopancreas and brain.The remaining fish measured personality traits(exploration,activity,boldness and sociability)and anxiety-like behavior.The results indicated:(1)The shelter group exhibited significantly higher growth rates compared to the none shelter group(35% increase in final weight,18% increase in specific growth rate,and 45% enhancement in weight gain rate).(2)Boldness was significantly greater in the shelter group(P<0.05;30.4% reduction in first entry time),while no significant differences were observed in exploration,activity,and sociability.(3)Shelter had no significant influence anxiety-like behavior,but cortisol levels were substantially lower in the shelter group(P<0.001).(4)Hepatopancreatic SOD and T-AOC were significantly reduced in the shelter group(P<0.05),whereas brain SOD activity displayed an opposite trend(P<0.05).The results suggest that the shelter minimally affects personality and anxiety-like behavior of S.sinensis.Placing shelters into aquaculture environments promotes fish growth,reduces stress levels,and modulates antioxidant capacity.These findings provide valuable insights for optimizing welfare-oriented practices in sustainable aquaculture systems.
作者
曾足仙
李武新
付世建
ZENG Zu-Xian;LI Wu-Xin;FU Shi-Jian(Laboratory of Evolutionary Physiology and Behavior,Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes/Animal Biology Key Laboratory of Chongqing Education Commission,Chongqing Normal University,Chongqing 401331,China)
出处
《水生生物学报》
北大核心
2025年第9期162-170,共9页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(32370509)资助。
关键词
隐蔽场所
鱼类福利
生长
抗氧化能力
个性
皮质醇
Shelters
Fish welfare
Growth
Antioxidant capacity
Personality
Cortisol
