摘要
为探究日粮中不同含量核黄素对日本沼虾线粒体生物合成及功能的影响,选用初重为(0.67±0.01)g的日本沼虾(Macrobrachium nipponense)作为实验对象,设置不同核黄素含量的3组半纯合饲料,其核黄素实际含量分别为3.91、169.61和326.66 mg/kg(分别为缺乏组、适宜剂量组与高剂量组),在室内循环系统中养殖10周。结果表明:169.61 mg/kg组的饲料效率、肝胰腺核黄素组Na^(+)-K^(+)-ATP酶、线粒体复合物Ⅲ活性及细胞色素C含量和细胞色素C氧化酶Ⅱ(cox-Ⅱ)基因表达量均显著高于3.91和326.66 mg/kg组(P<0.05),其肝胰腺ATP含量及cox-Ⅰ和ATP合成酶的基因表达量均显著高于3.91 mg/kg组(P<0.05),而与326.66 mg/kg组无显著差异(P>0.05);169.61 mg/kg核黄素组日增重系数以及肝胰腺琥珀酸脱氢酶活性显著高于326.66 mg/kg组(P<0.05),而与3.91 mg/kg组之间无显著差异(P>0.05);此外,326.66 mg/kg组的柠檬酸合酶活性显著高于另外两组(P<0.05),线粒体复合物Ⅴ活性显著高于3.91 mg/kg组(P<0.05),而3.91 mg/kg组的腺苷一磷酸/腺苷三磷酸比值显著高于另外两组(P<0.05)。由此可得,当饲料中核黄素含量为169.61 mg/kg时,能够提高日本沼虾的生长性能和饲料利用率,改善其线粒体生物合成与功能。
This study investigates the effects of different dietary riboflavin levels on the mitochondrial biogenesis and function of oriental river prawn(Macrobrachium nipponense)[average initial weight:(0.67±0.01)g].Three semi-purified diets with different riboflavin concentrations(3.91,169.61,and 326.66 mg/kg)were tested on prawns kept in an indoor recirculating water system for 10 weeks.The three treatments were designated as the deficient,optimal,and over-dosed groups,respectively.The results showed that in the 169.61 mg/kg riboflavin group,feed efficiency,hepatopancreatic Na^(+)-K^(+)-ATPase and mitochondrial complex Ⅲ activities,cytochrome C content,and Cytochrome C oxidase-Ⅱ(cox-Ⅱ)transcription were all significantly higher(P<0.05)than those in the 3.91 and 326.66 mg/kg groups.The hepatopancreatic ATP content and transcriptions of cox-Ⅰ and ATP synthase in the 169.61 mg/kg riboflavin group were all significantly higher than those of the 3.91 mg/kg group(P<0.05),but showed no significant difference with the 326.66 mg/kg group(P>0.05).However,the average daily gain,hepatopancreatic succinate dehydrogenase activity in the 169.61 mg/kg riboflavin group was significantly higher than that in the 326.66 mg/kg group(P<0.05),with no significant difference compared to the 3.91 mg/kg group(P>0.05).Additionally,the 326.66 mg/kg group showed significantly higher citrate synthase activity(P<0.05)than the other groups and a higher mitochondrial complex V activity than the 3.91 mg/kg group(P<0.05).Furthermore,the AMP/ATP ratio in the 3.91 mg/kg group was significantly higher compared with the other groups(P<0.05).These findings suggest that dietary inclusion of 169.61 mg/kg riboflavin can promote mitochondrial biogenesis and function in M.nipponense.
作者
刘子上
钱香钰
魏路瑶
刘波
孙存鑫
李向飞
LIU Zi-Shang;QIAN Xiang-Yu;WEI Lu-Yao;LIU Bo;SUN Cun-Xin;LI Xiang-Fei(Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;Freshwater Fisheries Research Center,Chinese Academy of Fishery Science,Wuxi 214081,China)
出处
《水生生物学报》
北大核心
2025年第9期107-115,共9页
Acta Hydrobiologica Sinica
基金
江苏现代农业(青虾)产业技术体系[JATS(2023)470]
国家虾蟹产业技术体系(CARS-48)资助。
关键词
核黄素
线粒体生物合成
线粒体功能
日本沼虾
Riboflavin
Mitochondrial biogenesis
Mitochondrial function
Macrobrachium nippone
