摘要
通过拥有独特分子重排机制的T细胞受体(TCR),成熟的T细胞能够特异性识别主要组织相容性复合体分子上的抗原。得益于二代测序技术和单细胞测序技术的发展,高通量TCR序列检测可运用于T细胞研究中。描述性分析、多样性和克隆性分析以及对抗原特异性序列的识别与鉴定等方法有助于研究者深入了解TCR组库信息,并已为感染、肿瘤和免疫学领域相关疾病的研究进展作出贡献。因而,TCR组库分析可应用于评估疾病进展情况并判断疾病预后效果,并且具备作为生物标志物辅助诊疗的潜力。此外,TCR组库分析还能够促进T细胞受体工程化T细胞(TCR-T)疗法的进一步发展。本综述就TCR组库分析及应用的最新进展进行阐述。
Mature T cells can specifically recognize antigens presented on molecules of the major histocompatibility complex via their T cell receptors(TCR),which undergo unique molecular restructuring mechanisms.Thanks to the development of next-gener-ation sequencing technology and single-cell sequencing technology,high-throughput detection of TCR sequences can be used in T cell research.Descriptive analysis,assessment of repertoire diversity and clonal expansion,together with identification and detection of antigen-specific sequences are all valuable for measuring the TCR repertoire.TCR sequencing and repertoire analysis have contributed to research progress in related diseases in the fields of infection,tumor and immunology.Consequently,TCR repertoire analysis can be employed to assess disease progression and prognosis,it also has the potential to serve as a diagnostic biomarker for treatment.Furthermore,TCR repertoire analysis may also facilitate further advancement of T cell receptor engineered T cell therapy(TCR-T).This review provides a comprehensive overview of the recent advances in the analysis and application of TCR repertoire.
作者
方智浩
龚方戚
FANG Zhihao;GONG Fangqi(Department of Cardiology,Children's Hospital,Zhejiang University School of Medicine,National Clinical Research Center for Child Health,Hangzhou 310052,China)
出处
《中国免疫学杂志》
北大核心
2025年第8期2012-2018,共7页
Chinese Journal of Immunology
基金
国家自然科学基金项目(81970434)。
