摘要
目的:探究VSTM1基因中rs612529 C/T和rs11669663 G/A是否与白细胞信号抑制受体-1(SIRL-1)的表达以及中国汉族人群系统性红斑狼疮(SLE)的发病风险相关。方法:纳入200例SLE患者和218例健康对照者(HC),收集SLE患者的相关实验室参数。通过Sanger测序技术对rs612529 C/T和rs11669663 G/A进行基因分型。利用流式细胞术评估外周血中性粒细胞和单核细胞上SIRL-1的表达情况。利用ELISA方法检测SLE患者相关自身抗体水平。结果:在SLE组和HC组,rs612529的C等位基因均与SIRL-1在单核细胞上的表达水平下降有关,SIRL-1蛋白水平从基因型CC到CT到TT逐渐升高。SLE患者中rs612529的C等位基因与血清抗dsDNA抗体水平升高有关(P<0.05)。未发现rs11669663 G/A基因型与SLE易感性的显著关联。结论:VSTM1 rs612529 C/T可能对SLE疾病活动度产生影响,且能够调节中国汉族人群单核细胞表面的SIRL-1表达。
Objective:To investigate whether the rs612529 C/T and rs11669663 G/A in VSTM1 gene are associated with leukocyte signaling inhibitory receptor-1(SIRL-1)expression and an increased risk for systemic lupus erythematosus(SLE)in a Han Chinese cohort.Methods:A total of 200 patients with SLE and 218 healthy controls(HC)were enrolled.Relevant laboratory characteris-tics of patients with SLE were also collected.Genotyping of rs612529 C/T and rs11669663 G/A were performed by Sanger sequencing technology.SIRL-1 expression was assessed in peripheral blood neutrophils and monocytes was detected by flow cytometry.Levels of autoantibodies associated with SLE were detected by ELISA.Results:In both SLE group and HC,the C allele of rs612529 was asso-ciated with a decreased expression level of SIRL-1 on monocytes,with a gradual increased in SIRL-1 protein level from the CC over the CT to the TT genotype.C allele of rs612529 was associated with higher serum anti-dsDNA antibody titers in patients with SLE(P<0.05).In the case of rs11669663 G/A,no significant association of genotypes with SLE susceptibility was detected.Conclusion:VSTM1 rs612529 C/T may contribute to SLE disease activity and regulate SIRL-1 expression on monocytes in the Han Chinese cohort.
作者
李静
袁佳仪
徐学静
徐臻
王岚
宗明
范列英
LI Jing;YUAN Jiayi;XU Xuejing;XU Zhen;WANG Lan;ZONG Ming;FAN Lieying(Department of Laboratory Medicine,East Hospital Affiliated To Tongji University,Shanghai 200120,China;Department of Laboratory Medicine,Affiliated Drum Tower Hospital of Nanjing Medical University,Nanjing 210008,China)
出处
《中国免疫学杂志》
北大核心
2025年第8期1975-1979,共5页
Chinese Journal of Immunology
基金
国家自然科学基金(81971535)。
