摘要
目的:探究睾酮对脂多糖(LPS)诱导的巨噬细胞M1型极化的影响。方法:CCK-8法检测LPS和睾酮对RAW264.7细胞活力的影响;光学显微镜观察细胞形态学变化;qRT-PCR检测巨噬细胞M1型极化基因TNF-α、IL-1β和IL-6的mRNA表达水平;免疫荧光和Western blot检测M1型巨噬细胞极化标志蛋白TNF-α和CD206的表达水平;ELISA检测条件培养基中上述炎症因子的分泌水平。结果:睾酮可降低M1型极化标志基因TNF-α、IL-1β和IL-6 mRNA和TNF-α蛋白表达水平,同时降低炎症相关因子的分泌。结论:睾酮可抑制LPS诱导的巨噬细胞向M1型极化表型转变。
Objective:To investigate effect of testosterone on polarization of M1 macrophages induced by lipopolysaccharide(LPS).Methods:CCK-8 method was used to detect effects of LPS and testosterone on RAW 264.7 cell viability.Morphological changes of cells were observed by optical microscope.mRNA expression levels of M1-type polarizing genes TNF-α,IL-1βand IL-6 in macro-phages were detected by qRT-PCR.Expression levels of M1-polarizing protein TNF-αand CD206 in macrophages were detected by immunofluorescence and Western blot.Secretion of inflammatory cytokines was detected by ELISA.Results:Testosterone could decrease mRNA expressions of TNF-α,IL-1βand IL-6 mRNA and protein expression of TNF-α.Finally,testosterone could decrease secretion of inflammation-related factors.Conclusion:Testosterone can inhibit LPS-induced transformation of macrophages to M1 polarization phenotype.
作者
田国良
张李栋
夏云冬
易龙
朱俊东
糜漫天
TIAN Guoliang;ZHANG Lidong;XIA Yundong;YI Long;ZHU Jundong;MI Mantian(Research Center for Nutrition and Food Safety/Chongqing Key Laboratory of Nutrition and Food Safety/Chongqing Medical Center of Nutrition,Faculty of Military Preventive Medicine,Army Medical University(Third Military Medical University),Chongqing 400038,China)
出处
《中国免疫学杂志》
北大核心
2025年第8期1940-1944,共5页
Chinese Journal of Immunology
