摘要
目的:探究杨梅素(Myr)对三硝基苯磺酸(TNBS)诱导的炎症性肠病(IBD)大鼠的作用和机制。方法:建立IBD大鼠模型,实验分为对照组、模型组、Myr低[28 mg(/kg·d),Myr-L、中56 mg(/kg·d),Myr-M]、高剂量[112 mg(/kg·d)Myr-H]组。对大鼠疾病活动指数(DAI)进行评分;试剂盒测定大鼠免疫功能指标及结肠长度;测定大鼠血清中IL-6、IL-17A、TNF-α、cAMP水平;检测结肠组织中MPO光密度值;HE染色观察大鼠结肠组织病理变化;流式细胞术测定Treg细胞比例。结果:与对照组比较,模型组结肠组织细胞排列紊乱,有大量炎症细胞浸润,溃疡现象明显,大量细胞坏死,黏膜水肿和结肠壁增厚,DAI评分、IL-6、IL-17A和TNF-α水平、脾脏指数、胸腺指数、MPO光密度值显著升高(P<0.05),结肠长度、cAMP水平、Treg细胞比例显著降低(P<0.05)。与模型组比较,Myr低、中、高剂量组大鼠结肠组织细胞排列较整齐、、cAMP膜水肿和结肠壁增厚减轻,炎症细胞浸润、细胞坏死及溃疡现象减少,DAI评分、IL-6、IL-17A和TNF-α水平、脾脏指数、胸腺指数、MPO光密度值逐渐降低(P<0.05),结肠长度、cAMP水平、Treg细胞比例显著升高(P<0.05)。结论:Myr可抑制炎症水平,调节免疫功能,对IBD大鼠发挥保护作用。
Objective:To explore the effect and mechanism of myricetin(Myr)on trinitrobenzenesulfonic acid(TNBS)-in-duced inflammatory bowel disease(IBD)in rats.Methods:IBD rat models were established and separated into Control group,Model group,low,medium and high dose Myr[28 mg(/kg·d),Myr-L,56 mg(/kg·d),Myr-M,112 mg(/kg·d),Myr-H]groups.The dis-ease activity index(DAI)of rats was scored.Immune function indicators and colon length were measured;reagent kits were applied to measure the levels of IL-6,IL-17A,TNF-αand cAMP in serum.MPO optical density values in colon tissue was detected.HE staining was applied to observe pathological changes in colon tissue.Flow cytometry was applied to determine the proportion of Treg cells.Results:Compared with the control group,the colon tissue cells in the model group were disordered in arrangement,with a large num-ber of inflammatory cell infiltrations,obvious ulcer phenomena,a large number of cell necrosis,mucosal edema and thickening of the colon wall,DAI score,IL-6,IL-17A and TNF-αlevels,spleen index,thymus index and MPO optical density value was increased sig-nificantly(P<0.05),colon length,cAMP level and the proportion of Treg cells were significantly decreased(P<0.05).Compared with the model group,the colonic tissue cells in the low-dose,medium-dose and high-dose Myr groups of rats were arranged more neatly,cAMP membrane edema and colonic wall thickening were alleviated,and inflammatory cell infiltration,cell necrosis and ulceration phenomena were reduced,DAI score,levels of IL-6,IL-17A and TNF-α,spleen index,thymus index,and MPO optical density val-ue were gradually decreased(P<0.05),while the colon length,cAMP level,and the proportion of Treg cells were significantly in-creased(P<0.05).Conclusion:Myr may inhibit inflammation levels,regulate immune function,and exert protective effects on IBD rats.
作者
周亚妮
王若敏
宋云
ZHOU Yani;WANG Ruomin;SONG Yun(Health Management School of Shangluo University,Shangluo 726000,China;Shangluo Health and Wellness Industry Research Institute,Shangluo 726000,China;Department of Immunology,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032,China)
出处
《中国免疫学杂志》
北大核心
2025年第8期1928-1932,1939,共6页
Chinese Journal of Immunology
基金
陕西省大学生创新创业训练计划项目(S202211396063)
商洛学院教学改革研究项目(24jyjx128)。
关键词
杨梅素
炎症性肠病
免疫功能
Myricetin
Inflammatory bowel disease
Immune function
