摘要
The persistence of bacteria in the root canal system is the primary cause of recurrent apical periodontitis.The adaptability of residual bacteria to changing environmental conditions is a key survival strategy of biofilms,often leading to endodontic treatment failure.DJK-5 is a protease-resistant,broad-spectrum D-enantiomeric peptide that degrades or prevents the accumulation of guanosine penta-and tetraphosphates,which are important for biofilm formation.We evaluated the effects of primary antimicrobial agents and nutrient conditions on the re-covery,metabolism,diversity,and composition of oral biofilms,and investigated how these factors affect the efficacy of DJK-5 and chlorhexidine(CHX)during re-exposure.Primary irrigants and nutrient conditions significantly influenced biofilm recovery,metabolic activity,diversity,and composition.Biofilm recovery was slower in nutrient-poor groups compared to nutrient-rich ones,and nutrient availability had the greatest effect on shaping both the diversity and composition of the biofilms.Water and DJK-5 groups showed similar biofilm diversity trends,while CHX generally led to lower diversity.Results indicate that primary irrigants and nutrient conditions significantly impact biofilm composition,diversity,and recovery.However,these changes did not compromise DJK-5’s effectiveness in killing of biofilm microbes during re-exposure of recovered biofilms.
