摘要
Periodontitis is a chronic inflammatory disease characterized by progressive alveolar bone resorption,and excessive reactive oxygen species(ROS)is a key factor to disease progression.Therefore,scavenging ROS to alleviate inflammation and promote bone regeneration are promising strategies to treat periodontitis.In this study,L-arginine(L-Arg)was used to modify mesoporous bioactive glass(MBG),forming L-Arg modified MBG(MBG@L-Arg),which showed effective ROS-scavenging and NO release properties in cells,and realize the protection and restoration of cell’s activity in ROS-rich microenvironment.Furthermore,MBG@L-Arg can induce macrophage polarization from M1 to M2 phenotype,and promote the osteogenic differentiation of MC3T3-E1 cells and human periodontal ligament stem cells(hPDLSCs).MBG@L-Arg also regulated anti-inflammatory and antioxidant systems by inhibiting the NF-κB signaling pathway and activating the Nrf2 signaling pathway.Besides,NO-PKG signaling pathway was also activated,further promoting bone regeneration.The in vivo results demonstrated that MBG@L-Arg can efficiently inhibit inflammation-induced tissue destruction and promote osteogenesis regeneration.The quantitative bone loss in MBG@L-Arg group was 1.03±0.05 mm,significantly lower than that of the periodontitis group(1.47±0.13 mm),implying that MBG@L-Arg can work as multi-functional materials for periodontal tissue regeneration.
基金
supported by the financial support from the National Natural Science Foundation of China(Nos.52163016&52463017)
Jiangxi Provincial Natural Science Foundation(20242BAB20394&20242BAB26161)
Major Discipline Academic and Technological leaders training programme of Jiangxi Province(20213BCJL22051)
Jiangxi Provincial Traditional Chinese Medicine Science and Technology project(2023B1235).
