摘要
目的 探索肠道菌群与结直肠癌之间的因果关系,通过孟德尔随机化(Mendelian randomization,MR)分析探索代谢物是否具有中介效应。方法 从公开数据库中获取473种肠道菌群、233种代谢物和结直肠癌的全基因组关联分析数据。将肠道菌群、代谢物分别与结直肠癌进行双向MR分析,随后用两步MR探查是否有特定的肠道菌群通过代谢物对结直肠癌的发生发展产生影响。结果 研究发现普雷沃菌属、阿托波菌科、费氏梭菌、洪加特氏菌属、微病毒球菌属、肽球菌纲和瘤胃球菌属7种肠道菌群的丰度与结直肠癌风险呈正相关;类芽孢杆菌目、口腔普雷沃菌、冷蛇菌属和琥珀酸弧菌科4种菌群丰度与结直肠癌风险呈负相关。通过两步法中介分析,证实中颗粒低密度脂蛋白中的甘油三酯与总脂质的比率可降低费氏梭菌作为结直肠癌发生的危险因素的影响。结论 本研究结果证实部分肠道菌群、代谢物与结直肠癌之间的因果关系并发现潜在交互链条,为结直肠癌的发病机制和防治研究提供思路。
Objective To explore the causal relationship between gut microbiota and colorectal cancer,and to investigate whether metabolites have a mediating effect through Mendelian randomization(MR)analysis.Methods Genome-wide association studies data of 473 gut microbiota,233 metabolites,and colorectal cancer were obtained from public databases.Bidirectional MR analyses were performed between gut microbiota,metabolites,and colorectal cancer,respectively.Subsequently,a two-step MR was used to explore whether specific gut microbiota affect the occurrence and development of colorectal cancer through metabolites.Results The study found that the abundances of 7 gut microbiota were positively correlated with the risk of colorectal cancer:Alloprevotella,Atopobiaceae,Clostridium S felsineum,Hungatella,Microvirga,Peptococcia and Ruminococcus A;The abundances of 4 gut microbiota were negatively correlated with the risk of colorectal cancer:Paenibacillales,Prevotella buccae,Psychroserpens and Succinivibrionaceae.Through the two-step mediation analysis,it was confirmed that the ratio of Triglycerides to total lipids ratio in medium very low-density lipoprotein could reduce the impact of Clostridium S felsineum as a risk factor for the occurrence of colorectal cancer.Conclusion The results of this study confirm the causal relationship between some gut microbiota,metabolites,and colorectal cancer,and discover potential interaction chains,providing ideas for the pathogenesis and prevention of colorectal cancer.
作者
王佳伟
洪中华
尹和宅
WANG Jiawei;HONG Zhonghua;YIN Hezhai(Department of Anorectal Surgery, Jiaxing Traditional Chinese Medicine Hospital)
出处
《中国现代医生》
2025年第24期55-62,共8页
China Modern Doctor
关键词
肠道菌群
代谢物
孟德尔随机化
结直肠癌
Gut microbiota
Metabolites
Mendelian randomization
Colorectal cancer
