摘要
目的 探讨长链非编码RNA(long intergenic non-coding RNA,RNA LINC)00665、糖类抗原153(carbohydrate antigen153,CA153)联合检测在乳腺癌诊断及预后评估中的价值。方法 选取2023年1月至12月九江市第一人民医院收治的50例乳腺癌患者纳入乳腺癌组,选取同期在本院接受治疗的50例乳腺纤维腺瘤患者纳入乳腺纤维腺瘤组,另选取同期在本院体检的50名女性健康体检者为对照组。收集纳入者的临床资料,分别采用实时荧光定量聚合酶链反应法与电化学发光法检测LINC00665和CA153水平。比较三组纳入者的LINC00665、CA153表达水平差异,采用Spearman相关性分析探讨血清LINC00665和CA153表达相关性;受试者操作特征曲线(receiver operating characteristic curve,ROC曲线)分析LINC00665、CA153及二者联合检测对乳腺癌的诊断价值;多因素回归分析探讨影响乳腺癌预后的因素。结果 与对照组比较,乳腺癌组患者的血清LINC00665和CA153表达水平较高,乳腺纤维腺瘤组患者的血清LINC00665表达水平较高;与乳腺纤维腺瘤组比较,乳腺癌组患者的血清LINC00665和CA153表达水平较高,差异有统计学意义(P<0.05)。血清LINC00665、CA153表达与乳腺癌的TNM分期、病理类型、淋巴结转移相关(P<0.05)。Spearman相关性分析结果显示,乳腺癌患者的血清LINC00665与CA153表达呈线性正相关(r=0.651,P<0.05)。ROC曲线分析结果显示LINC00665与CA153联合诊断价值高于单独诊断(P<0.05)。多因素回归分析结果显示LINC00665、CA153、TNM分期、淋巴结转移是影响乳腺癌患者预后的独立危险因素(P<0.05)。结论 乳腺癌患者的血清LINC00665、CA153表达水平较高,与TNM分期、病理类型、淋巴结转移相关,可用于乳腺癌诊断及预后评估。
Objective To explore the value of the combined detection of long intergenic non-coding RNA(RNA LINC)00665 and carbohydrate antigen 153(CA153)in the diagnosis and prognosis assessment of breast cancer.Methods 50 breast cancer patients admitted to Jiujiang First People’s Hospital were selected to be included in breast cancer group from January to December 2023,50 breast fibroadenoma patients treated in the hospital during the same period of time were selected to be included in breast fibroadenoma group,and another 50 female healthy individuals undergoing physical examination in the hospital during the same period of time were selected to be control group.The clinical data of the enrolled patients were collected,and the levels of LINC00665 and CA153 were detected by reverse transcription quantitative polymerase chain reaction and electrochemiluminescence respectively.The differences in the expression levels of LINC00665 and CA153 among three groups were compared,and the correlation between the expression levels of serum LINC00665 and CA153 was analyzed by Spearman correlation analysis;The diagnostic value of LINC00665,CA153 and the combined detection of the two in the diagnosis of breast cancer was analyzed by receiver operating characteristic(ROC)curve,and the factors affecting the prognosis of breast cancer were analyzed by multifactorial regression.Results Compared with control group,serum LINC00665 and CA153 expression levels were higher in breast cancer group,and serum LINC00665 expression levels were higher in breast fibroadenoma group.Serum LINC00665 and CA153 expression was correlated with TNM stage,pathological type,and lymph node metastasis of breast cancer(P<0.05).Spearman correlation analysis showed that there was a linear positive correlation between serum LINC00665 and CA153 expression in patients with breast cancer(r=0.651,P<0.05).ROC curve analysis showed that the value of combined diagnosis of LINC00665 and CA153 was higher than that of individual diagnosis(P<0.05).The results of multifactorial regression analysis showed that LINC00665,CA153,TNM stage,and lymph node metastasis were independent risk factors affecting the prognosis of breast cancer patients(P<0.05).Conclusion The higher expression levels of serum LINC00665 and CA153 in breast cancer patients were correlated with TNM stage,pathologic type,and lymph node metastasis,which can be used in the diagnosis and prognosis evaluation of breast cancer.
作者
陈晓宇
华琳
吴建鑫
CHEN Xiaoyu;HUA Lin;WU Jianxin(Department of Laboratory Medicine,Jiujiang First People’s Hospital,Jiujiang 332000,Jiangxi,China)
出处
《中国现代医生》
2025年第24期17-20,94,共5页
China Modern Doctor
基金
江西省九江市科技计划项目(S2023ZDYFN733)。
