摘要
目的:探讨胃镜活检组织核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、含半胱氨酸的天冬氨酸蛋白水解酶1(Caspase-1)表达与胃癌分化程度及预后的关系。方法:选择接受胃癌根治性手术的胃癌患者159例为胃癌组,并选取同期胃良性疾病患者97例为对照组。胃癌组分别采集肿瘤组织及癌旁正常组织标本,对照组采集病变组织标本。应用实时荧光定量聚合酶链式反应(RT-qPCR)细胞焦亡标志物NLRP3、Caspase-1及凋亡相关斑点样蛋白(ASC)mRNA的表达水平。术后通过定期随访收集患者生存状态,分别纳入死亡组和幸存组。比较不同组别间NLRP3、Caspase-1、ASC mRNA的表达差异,分析胃癌患者预后的影响因素。结果:与对照组活检组织比较,胃癌组织和癌旁正常组织NLRP3、Caspase-1、ASC mRNA表达显著上调,且胃癌组织NLRP3、Caspase-1、ASC表达较癌旁正常组织进一步上调(均P<0.05)。随访期间死亡患者95例。与幸存组比较,死亡组中低分化占比、T 4分期占比及NLRP3、Caspase-1 mRNA相对表达量增加(均P<0.05)。多因素Cox回归分析显示,肿瘤中低分化及NLRP3 mRNA高表达、Caspase-1 mRNA高表达是胃癌患者预后的独立危险因素(均P<0.05)。中低分化程度胃癌患者NLRP3 mRNA相对表达量高于高分化患者(均P<0.05)。结论:NLRP3和Caspase-1在胃镜活检肿瘤组织中呈高表达,且中低分化患者表达高于低分化患者,两者均与不良预后密切相关。
Objective:To investigate the relationship between the expressions of nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3),cysteine-aspartic acid protease-1(Caspase-1)in gastric endoscopic biopsy tissues and the degree of gastric cancer differentiation as well as prognosis.Methods:A total of 159 patients with gastric cancer who underwent radical gastrectomy were selected as the gastric cancer group,and 97 patients with benign gastric diseases during the same period were selected as the control group.Tumor tissues and adjacent normal tissues were collected from the gastric cancer group,while lesion tissue specimens were collected from the control group.RT-qPCR was used to detect the expression levels of NLRP3,Caspase-1 and apoptosis-associated speck-like protein containing a CARD(ASC)mRNA.Postoperatively,the survival status of patients was collected through regular follow-up,and they were respectively included in the death group and the survival group.The expression differences of NLRP3,Caspase-1 and ASC mRNA among different groups were compared,and the influencing factors of the prognosis of gastric cancer patients were analyzed.Results:Compared with the biopsy tissues of the control group,the mRNA expressions of NLRP3,Caspase-1 and ASC in gastric cancer tissues and adjacent normal tissues were significantly upregulated,and the mRNA expressions of NLRP3,Caspase-1 and ASC in gastric cancer tissues were further upregulated compared with those in adjacent normal tissues(all P<0.05).During the follow-up period,95 patients died.Compared with the survival group,the proportions of moderately and poorly differentiated and T 4 stage,and the relative expression levels of NLRP3 and Caspase-1 mRNA were increased(all P<0.05).Multivariate Cox regression analysis showed that moderately and poorly differentiated tumors and high expressions of NLRP3 mRNA,Caspase-1 mRNA were independent risk factors for the prognosis of gastric cancer patients(all P<0.05).The relative expression level of NLRP3 mRNA in patients with moderately and poorly differentiated gastric cancer was higher than that in patients with highly differentiated tumors(all P<0.05).Conclusion:NLRP3 and Caspase-1 are highly expressed in the tumor tissues obtained from gastroscopy biopsies,and their expressions are higher in patients with moderate and poorl differentiation than in those with high differentiation.Both are closely related to poor prognosis.
作者
袁翠
何银艳
卢伟
刘佳
程为
张培
YUAN Cui;HE Yinyan;LU Wei;LIU Jia;CHENG Wei;ZHANG Pei(Endoscopy Center,the Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China;Operating Room,the Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China)
出处
《陕西医学杂志》
2025年第9期1264-1269,1284,共7页
Shaanxi Medical Journal
基金
湖南省卫生健康委员会科研项目(20212432)。
关键词
胃癌
核苷酸结合寡聚化结构域样受体蛋白3
含半胱氨酸的天冬氨酸蛋白水解酶1
凋亡相关斑点样蛋白
细胞焦亡
分化程度
预后
Gastric cancer
Nucleotide-binding oligomerization domain-like receptor protein 3
Cysteine-aspartic acid protease-1
Apoptosis-associated speck-like protein containing a CARD
Pyroptosis
Degree of differentiation
Prognosis
