摘要
目的:基于Cu/Zr-IM1AA纳米酶构建抗坏血酸(Ascorbic Acid,AA)比色传感平台,实现AA的高灵敏比色检测与图像可视化分析。方法:设计Cu/Zr-IM1AA纳米酶催化反应体系,结合比色检测法(基于吸光度变化)和图像检测法(基于图像参数变化)建立双模式快速分析方法。结果:比色检测法在AA浓度为0~0.48 mmol·L^(-1)时表现出良好的线性,线性方程为y=-0.400 3x+0.272 3,相关系数为0.998 4,检出限为6.2 nmol·L^(-1)。图像检测法在AA浓度为0~120μmol·L^(-1)时表现出良好的线性,线性方程为lgx=0.005 733z+0.726,R^(2)为0.978 5,检出限为47.6 nmol·L^(-1)。结论:两种方法均具备高灵敏性与重现性,其中比色检测适用于痕量AA的检测,而图像检测则为AA的快速可视化分析提供了便捷的手段。
Objective:To construct an Ascorbic Acid(AA)colorimetric sensing platform based on Cu/Zr-IM1AA nanoenzyme to achieve highly sensitive colorimetric detection and image visualization of AA.Method:A Cu/Zr-IM1AA nanoenzyme catalytic reaction system was designed,and a dual-mode rapid analysis method was established by combining colorimetric detection method(based on absorbance changes)and image detection methods(based on image parameter changes).Result:The results of the colorimetric detection method were 0 mmol·L^(-1) to 0.48 mmol·L^(-1),the linear equation is y=-0.4003x+0.2723,the correlation coefficient is 0.9984,and the limit of detection is 6.2 nmol·L^(-1).The image detection method was 0μmol·L^(-1) to 120μmol·L^(-1),the linear equation is lgx=0.005733z+0.726,the correlation coefficient is 0.9785,and the detection limit is 47.6 nmol·L^(-1).Conclusion:Both methods have high sensitivity and reproducibility,among which colorimetric detection is suitable for the detection of trace AA,while image detection provides a convenient means for rapid visual analysis of AA.
作者
于娇娇
申凯龙
刘梅
本莲芳
刘继超
YU Jiaojiao;SHEN Kailong;LIU Mei;BEN Lianfang;LIU Jichao(College of Petrochemical Engineering,Lanzhou Petrochemical University of Vocational Technology,Lanzhou 730060,China;Hengyi Petrochemical Co.,Ltd.-Caprolactam Company,Hangzhou 311247,China)
出处
《现代食品》
2025年第15期215-220,共6页
Modern Food
基金
甘肃省高等学校创新基金项目“金属有机骨架材料在环境中内分泌干扰物富集检测中的应用”(2022A-214)
甘肃省青年人才(团队)项目“基于共价有机框架材料的样品前处理技术在肉类食品安全分析中的应用研究”(2025QNTD10)。
关键词
纳米酶
抗坏血酸(AA)
比色检测
图像检测
nanoenzymes
Ascorbic Acid(AA)
colorimetric detection
image detection
