摘要
目的:探讨病原灭活技术(PITs)对机采血小板(PLT)储存期间的数量、形态及功能的影响,为PLT病原灭活提供一定的理论基础。方法:随机选取自愿无偿献血者2个治疗量机采PLT30份作为研究对象,将每份(两袋PLT)中的一袋PLT作为实验组,另一袋作为对照组。对照组PLT不进行病原灭活处理,实验组PLT进行病原灭活处理,分别在PLT储存的第0、2、6、8天检测两组PLT的聚集功能与活性变化、PLT计数、PLT分布宽度(PDW)、平均PLT体积(MPV),实验重复3次。结果:第2、6、8天,两组PLT计数、PDW、MPV与第0天比较,差异无统计学意义(P>0.05);第6、8天,对照组PLT膜糖蛋白α2β1整合素(GPIa/Ⅱa)表达低于第0天,第2、6、8天,实验组低于第0天,第8天,实验组高于对照组,差异均具有统计学意义(P<0.05);第2、6、8天,两组PLT膜糖蛋白Ⅵ(GP6)表达均低于第0天,第6天,实验组低于对照组,差异均具有统计学意义(P<0.05);第2、6、8天,两组PLTP-选择素(CD62p)表达率均高于第0天,CD62p再表达率低于第0天,第6天,实验组CD62p表达率高于对照组,第2、8天,实验组CD62p再表达率高于对照组,第6天,实验组CD62p再表达率低于对照组,差异均具有统计学意义(P<0.05)。结论:PITs对机采PLT储存期间的数量及形态影响不大,但对储存期间PLT的聚集功能有一定影响;并有可能导致输注储存期为6~8 d的病原灭活PLT的黏附、聚集功能不良。由此看来,PITs虽然可以降低PLT储存期间的病原感染发生率,同时也会在一定程度上影响PLT长期储存的活性和功能,因此如何平衡PLT输注质量和避免PLT病原感染之间的关系将是新的挑战。
Objective To explore the effects of pathogen inactivation technology(PITs)on the quantity,morphology and function of apheresis platelets(PLT)during storage,and to provide a certain theoretical basis for pathogen inactivation of PLT.Methods Two therapeutic doses of machine-collected PLT from voluntary unpaid blood donors were randomly selected as the research subjects.One bag of PLT from each dose(two bags of PLT)was used as an experimental group,and the other bag was used as a control group.The PLT in the control group was not subjected to pathogen inactivation treatment,while the PLT in the experimental group was subjected to pathogen inactivation treatment.The aggregation function,activity changes,PLT count,PLT distribution width(PDW),and average PLT volume(MPV)of the PLT in the two groups were detected on the 0,2,6,and 8 days of PLT storage,respectively.The experiments were repeated three times.Results On the 2nd,6th,and 8th days,there was no statistically significant difference in PLT count,PDW,and MPV between the two groups compared with those on the 0th day(P>0.05).On the 6th and 8th days,the expression of PLT membrane glycoproteinα2β1 integrin(GPIa/Ⅱa)in the control group was lower than that on the 0th day.On the 2nd,6th,and 8th days,it was lower in the experimental group than that on the 0th day.On the 8th day,the expression in the experimental group was higher than that in the control group.The differences were all statistically significant(P<0.05).On the 2nd,6th,and 8th days,the expression of PLT membrane glycoproteinⅥ(GP6)in both groups was lower than that on the 0th day.On the 6th day,the expression in the experimental group was lower than that in the control group,and the differences were statistically significant(P<0.05).On the 2nd,6th and 8th days,the expression rate of PLT p-selectin(CD62p)in both groups was higher than that on the 0th day,and the re-expression rate of CD62p was lower than that on the 0th day.On the 6th day,the expression rate of CD62p in the experimental group was higher than that in the control group.On the 2nd and 8th days,the re-expression rate of CD62p in the experimental group was higher than that in the control group.On the 6th day,the re-expression rate of CD62p in the experimental group was lower than that in the control group.The differences were all statistically significant(P<0.05).Conclusion PITs have little influence on the quantity and form of mechanical PLT during storage,but they do have a certain impact on the aggregation function of PLT during storage.It may also lead to poor adhesion and aggregation functions of pathogen-inactivated PLTS with a storage period of 6 to 8 days after infusion.From this perspective,although PITs can reduce the incidence of pathogen infection during PLT storage,it will also affect the activity and function of PLT long-term storage to a certain extent.Therefore,how to balance the quality of PLT infusion and avoid pathogen infection of PLT will be a new challenge.
作者
祝丽双
张袁丹
王浩
ZHU Lishuang;ZHANG Yuandan;WANG Hao(Department of Blood Transfusion,Fifth Clinical Medical College of Henan University of Chinese Medicine/Zhengzhou People's Hospital,Henan Zhengzhou 450000;Ophthalmology Department,Henan Provincial People's Hospital,Henan Zhengzhou 450000)
出处
《深圳中西医结合杂志》
2025年第10期20-22,30,I0008,I0009,共6页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
基金
河南省医学科技攻关计划项目(LHGJ20210699)。
关键词
机采血小板
病原灭活技术
血小板形态
血小板功能
Apheresis platelets
Pathogen inactivation technology
Platelet morphology
Platelet function
